论文部分内容阅读
目的研究抗癌灵正丁醇提取部位(以下简称KAL)对多种肿瘤细胞的抑制率以及时效和量效关系。方法采用MTT法考察KAL对肿瘤细胞B-16、SGC-7901、Colo320、PC-3、HL60的抑制率以及IC50;通过观察KAL对肿瘤细胞SGC-7901和PC-3生长的影响,考察其时效和量效关系。结果 KAL对肿瘤细胞B-16、SGC-7901、Colo320、PC-3、HL60的IC50分别为:(21.05±4.31),(13.43±4.78),(15.17±3.82),(26.39±5.76),(25.78±4.40)μg·mL-1,且在抑制肿瘤细胞过程中呈现明显的时效关系和量效关系。结论 KAL对肿瘤细胞B-16、SGC-7901、Colo320、PC-3、HL60具有显著抑制作用。
Objective To study the anti-cancer Ling n-butanol extraction site (hereinafter referred to as KAL) inhibition of a variety of tumor cells and the relationship between aging and dose-effect. Methods The inhibitory rate and IC50 of KAL on tumor cells B-16, SGC-7901, Colo320, PC-3 and HL60 were determined by MTT assay. The effect of KAL on the growth of SGC-7901 and PC-3 cells was investigated. And dose-effect relationship. Results The IC50 of KAL on tumor cells B-16, SGC-7901, Colo320, PC-3 and HL60 were (21.05 ± 4.31), (13.43 ± 4.78), (15.17 ± 3.82) and (26.39 ± 5.76) 25.78 ± 4.40) μg · mL-1, and showed significant time-effect and dose-effect relationship in inhibiting tumor cells. Conclusion KAL has a significant inhibitory effect on tumor cells B-16, SGC-7901, Colo320, PC-3 and HL60.