论文部分内容阅读
目的 研究诱导型一氧化氮合酶(iNOS)与血红素氧合酶 l(HO 1)在肠易激综合征(IBS)患者结肠中 的表达,探讨气体信使分子一氧化氮(NO)和一氧化碳(CO)在IBS发病机制中的可能作用。 方法 采用免疫组织 化学EnVision和SABC染色法,对符合罗马Ⅱ诊断标准的34例IBS患者肠镜活检标本分别检测结肠黏膜组织中 iNOS和HO 1的阳性细胞,并以16名健康者做对照。 结果 iNOS和HO 1主要表达在结肠黏膜上皮细胞,IBS患 者表达强于对照组(P<0.001),且HO 1和iNOS表达呈正相关(r=0.5744,P=0.0004)。 结论 iNOS和HO 1在 IBS患者结肠中的表达异常,表明CO和NO在IBS等肠动力性疾病的发病机制中可能起重要作用。
Objective To study the expression of inducible nitric oxide synthase (iNOS) and heme oxygenase 1 (HO 1) in the colon of patients with irritable bowel syndrome (IBS) and to explore the role of gas messenger molecule nitric oxide (NO) and carbon monoxide (CO) in the pathogenesis of IBS may play a role. Methods Immunohistochemistry EnVision and SABC staining were used to detect the positive cells of iNOS and HO 1 in colonic mucosa of 34 IBS patients who meet the diagnostic criteria of Rome Ⅱ and 16 healthy controls. Results The expressions of iNOS and HO 1 were mainly in colonic epithelial cells. The expression of iNOS and HO 1 in IBS patients was stronger than that in the control group (P <0.001). The expression of HO 1 and iNOS was positively correlated (r = 0.5744, P = 0.0004). Conclusions The abnormal expressions of iNOS and HO 1 in the colon of patients with IBS suggest that CO and NO may play important roles in the pathogenesis of intestinal motility disorders such as IBS.