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以肾综合征出血热（ＨＦＲＳ）患者血清中提取的γ球蛋白与ＨＦＲＳ病毒抗原形成的免疫复合物（ＩＣ）为模型，抗人μ链和γ链单克隆抗体（ＭＡｂ）为包被抗体，辣根过氧化物酶标记的抗－ＨＦＲＳ病毒组特异性ＭＡｂ为指示抗体，建立了检测ＨＦＲＳ特异性循环ＩＣ（包括ＩｇＭ型和ＩｇＧ型）的ＥＬＩＳＡ捕捉法，并初步用于ＨＦＲＳ急性期患者血清标本的检测。结果表明该法特异，敏感，简便，可用于ＨＦＲＳ发病机理及免疫功能的研究。
The immunocomplexes (ICs) of γ globulin and HFRS virus antigen extracted from the serum of patients with hemorrhagic fever with renal syndrome (HFRS) were used as the model. Anti-human μ-chain and γ-chain monoclonal antibody (MAb) The horseradish peroxidase-labeled anti-HFRSV group-specific MAb is an indicator antibody, and an ELISA capture method for detecting HFRS-specific circulating ICs (including IgM and IgG types) has been established and is initially used in patients with HFRS acute phase serum Specimen testing. The results show that the method is specific, sensitive and simple, and can be used to study the pathogenesis and immune function of HFRS.