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近年来,在细胞治疗和再生医学领域,自体或异体细胞移植治疗疾病正在成为现实.骨髓间充质干细胞具有分化成多种细胞的潜能,已被广泛用于各种疾病的研究和治疗.活体追踪移植细胞,检测移植细胞的生存及功能状态对于评价移植治疗效果至关重要.目前,利用磁共振对比剂超顺磁性氧化铁颗粒(SPIO),活体追踪和监测标记细胞已被广泛用于动物实验研究和一些临床疾病诊断.但MRI信号不能显示移植细胞在体内的生物学特征.本研究中,对食蟹猴骨髓间充质干细胞体外标记Molday ION rhodamine-B~(TM)(MIRB),探讨MIRB标记后cMSCs的细胞生物学特性,以及脑内移植后的活体MRI影像学及组织学追踪.结果表明,MIRB具有生物组织相容性,能高效标记cMSCs,可用于体内多模式追踪移植细胞,为利用MIRB追踪和检测移植细胞,以及干细胞移植治疗机制的研究提供资料.
In recent years, autologous or allogeneic cell transplantation has become a reality in the field of cell therapy and regenerative medicine, and has the potential to differentiate into many types of cells and has been widely used in the research and treatment of various diseases. Tracing the transplanted cells to detect the survival and functional status of the transplanted cells is crucial for assessing the efficacy of the transplanted treatment.Currently, the use of magnetic resonance contrast agent superparamagnetic iron oxide particles (SPIO), in vivo tracking and monitoring of labeled cells, has been widely used in animals Experimental studies and diagnosis of some clinical diseases.But MRI signals can not show the biological characteristics of the transplanted cells in vivo.In this study, C57BL / 6 mice were treated with Molday ION rhodamine-B (TM) (MIRB) To investigate the biological characteristics of c-MSCs after MIRB labeling and the imaging of living grafts after brain transplantation and histological tracing.Results show that MIRB has biocompatibility and is capable of labeling cMSCs efficiently and can be used to track multiple transplanted cells in vivo , For the use of MIRB tracking and detection of transplanted cells, and stem cell transplantation mechanism of treatment to provide information.