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目的:研究新基因Mip5(GenBank登录号AY553870)的特性及其在生理或病理状态下的表达变化。方法:运用BLAST,spidey,psort,ClustalW等生物信息学方法对Mip5的相应特性进行分析,并运用逆转录聚合酶链式反应(RT-PCR)研究基因Mip5的表达。结果:生物信息学分析显示Mip5位于第13号染色体,其开放读码框为909bp,编码302个氨基酸,含有8个外显子和7个内含子。Mip5蛋白含有6个Kelch结构。RT-PCR检测发现正常时,Mip5在心,脑,肾等组织中表达丰度较高,而在骨骼肌和肝脏组织未能检测到其表达;缺血/再灌注损伤后不同时间心肌组织中Mip5的表达较假手术组明显升高,在再灌注后3h达高峰,12h恢复至基线水平。结论:上述结果表明Mip5为缺血/再灌注相关基因,可能在心肌缺血病理过程中发挥作用。
Objective: To study the characteristics of new gene Mip5 (GenBank accession number AY553870) and its expression in physiological or pathological conditions. Methods: The bioinformatics methods such as BLAST, spidey, psort and ClustalW were used to analyze the characteristics of Mip5 and the expression of Mip5 gene was analyzed by reverse transcription polymerase chain reaction (RT-PCR). Results: Bioinformatics analysis showed that Mip5 was located on chromosome 13 with an open reading frame of 909 bp and encoded 302 amino acids. It contained 8 exons and 7 introns. The Mip5 protein contains six Kelch structures. MTP5 was found to be highly expressed in heart, brain and kidney tissues, but not in skeletal muscle and liver tissue. Mip5 expression in myocardium at different time points after ischemia / reperfusion injury was detected by RT-PCR. The expression was significantly higher than that in sham operation group, peaked at 3h after reperfusion and returned to baseline at 12h. Conclusion: The above results indicate that Mip5 is a gene related to ischemia / reperfusion, which may play a role in the pathological process of myocardial ischemia.