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目的：介绍一种测定人体尿液诱变性的方法。方法：将健康人外周血置于透析袋中于待测尿样中透析一定时间后进行血液培养，通过分析淋巴细胞姐妹染色单体互换频率（ＳＣＥ）的变化来评价被测尿样潜在的致诱变性。结果：实验结果表明，透析过程不会引起ＳＣＥ频率变化（３．１４～３．２４ＳＣＥ／细胞，Ｐ＞０．０５，ｔ检验），而吸烟者，环氧乙烷作业工人，接受化疗的肿瘤患者尿液可引起ＳＣＥ明显升高（Ｐ＜０．０１，ｔ检验）分别达到４．１２～４．２８ＳＣＥ／细胞，４．５１～４．６９ＳＣＥ／细胞，１３．４０～１７．４７ＳＣＥ／细胞。结论：该方法在评价环境中诱变物／致癌物中具有一定的应用意义。
Objective: To introduce a method to determine the mutagenicity of human urine. Methods: Peripheral blood of healthy persons was placed in dialysis bags for dialysis for a certain period of time in the urine samples to be tested. Blood culture was performed. Changes in lymphocyte sister chromatid exchange frequency (SCE) were analyzed to evaluate the potential of the tested urine samples. Mutagenicity. Results: The experimental results show that the dialysis process does not cause changes in SCE frequency (3.14 to 3.24 SCE/cell, P>0.05, t-test), whereas smokers, ethylene oxide workers, tumors undergoing chemotherapy Urine in the patient can cause a significant increase in SCE (P<0.01, t-test) 4.12 to 4.28 SCE/cell, 4.51 to 4.69 SCE/cell, and 13.40 to 17.47 SCE/cell, respectively. . Conclusion: This method has certain application significance in evaluating environmental mutagen/carcinogens.