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目的 了解流行于山东境内HIV - 1毒株的亚型分布及变异情况 ,分析其来源并推测其流行趋势。方法 采集了 2 5份HIV - 1抗体阳性感染者的全血分离单核细胞 (PBMC) ,提取前病毒DNA ,经nested -PCR扩增HIV - 1膜蛋白 (env)基因的C2 -V3区并进行序列测定和亚型分析。结果 2 5份HIV - 1阳性感染者的PBMC样品中扩增到 2 4份可用于序列测定的HIV - 1env基因片段 ,经序列测定和基因分析鉴别出共有 5种HIV - 1M亚群基因亚型 :A、B、C、E和B′亚型。 13名有偿献血人员均为B′(泰国B)亚型 ,10名回国劳工及 1名回国劳工的女性配偶中存在A、B、C、E四种亚型。结论 山东省HIV - 1各种亚型毒株感染情况复杂 ,因此应加强对献血者及回国劳工等高危人群的检测和控制 ,以防HIV - 1各亚型毒株在全省的蔓延
Objective To understand the distribution and variation of subtypes of HIV - 1 strains endemic to Shandong Province, analyze their sources and speculate their epidemiological trends. Methods Twenty - five whole blood mononuclear cells (PBMC) from HIV - 1 positive patients were collected and the proviral DNA was extracted. The C2 - V3 region of HIV - 1 membrane protein (env) gene was amplified by nested - PCR Sequencing and subtype analysis were performed. Results A total of 24 HIV - 1env gene fragments that could be used for sequence determination were amplified from PBMC samples from 25 HIV - 1 positive individuals. A total of 5 HIV - 1 subgroup subtypes were identified by sequencing and gene analysis : A, B, C, E and B ’subtypes. Thirteen A, B, C and E subtypes were found among the 13 female blood donors who were both B ’(Thai B) subtype, 10 returning workers and one returning worker. Conclusion The infection of HIV - 1 subtypes in Shandong Province is complicated. Therefore, the detection and control of high risk groups such as blood donors and returning workers should be strengthened so as to prevent the spread of HIV - 1 subtypes in the whole province