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血小板激活因子(PAF)是具有多种生物功能的磷酯,正常人及轻症哮喘患者吸入PAF 后可引起支气管收缩,PAF 起效快(2～3min),作用时间短(15～45min),部分人可发生颜面潮红和温热感。为了探讨PAF 诱发人类支气管收缩的机制,本文对年龄18～36岁的3例正常人和4例轻症哮喘患者进行乙酰甲胆碱(1次)和FAF(4次)激发试验,每例共试验5次,每次间隔至少2天。实验前先作预处理,分别雾化吸入磷酸盐缓冲液(PBS,作为对照)、硫酸阿托品(1.5mg)和口服扑尔敏(8mg)或消炎痛(50mg)。雾化吸入PAF 的最低浓度为0.1μg/ml,再以5～10詹浓度递增到1000μg/ml。以肺功能(FEV_1,SGaW 及V30P 等)为指标,观察每次试验前后的变化。V30p 即部分呼气流速-容量曲线上从RV 位取30%肺量的流速。吸入
Platelet activating factor (PAF) is a kind of phospholipid which has a variety of biological functions. PAF can cause bronchoconstriction in normal and mild asthmatic patients. PAF has a rapid onset of action (2-3 min), short acting time (15-45 min) Some people may be facial flushing and warm feeling. In order to investigate the mechanism of PAF-induced bronchoconstriction in human beings, three normal and four mild asthma patients aged 18-36 years were challenged with methacholine (1 time) and FAF (4 times) Test five times, each interval of at least 2 days. Pretreatment was performed prior to the experiment. Phosphate buffered saline (PBS as a control), atropine sulfate (1.5 mg) and oral chlorpheniramine (8 mg) or indomethacin (50 mg) were separately inhaled. The minimum concentration of aerosolized PAF was 0.1μg / ml, and then increased to 1000μg / ml with 5-10% concentration. Lung function (FEV_1, SGaW and V30P, etc.) was used as an index to observe the changes before and after each test. V30p is the partial expiratory flow rate - the flow rate on the volume curve that takes 30% of the spit from the RV. Inhalation