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目的:探讨喘平方防治支气管哮喘的作用机制。方法:取白化豚鼠60只,随机分为正常组、模型组、麻黄组(0.20 g.kg-1)、洋金花组(0.07 g.kg-1)、喘平方组(0.32 g.kg-1)、地塞米松组(7.5×10-4mg.kg-1),通过对豚鼠ip卵蛋白致敏并雾化吸入激发,建立实验性哮喘豚鼠模型;治疗组自注射第14天起每天ig给药1次,连续7 d,正常组及模型组给予生理盐水。观察豚鼠的一般情况,豚鼠肺泡灌洗液中免疫球蛋白E(IgE),转化生长因子β1(TGF-β1),肿瘤坏死因子α(TNF-α)的变化,肺组织病理情况。结果:各组豚鼠肺泡灌洗液中IgE,TGF-β1,TNF-α分别为:正常组IgE(68.25±5.92)mg.L-1,TGF-β1(78.72±10.92)ng.L-1,TNF-α(388.02±55.61)ng.L-1;模型组IgE(137.28±14.38)mg.L-1,TGF-β1(172.39±14.04)ng.L-1,TNF-α(752.76±51.25)ng.L-1;喘平方组IgE(76.35±6.22)mg.L-1,TGF-β1(115.76±9.17)ng.L-1,TNF-α(569.32±39.7)ng.L-1;哮喘组及喘平方组豚鼠肺泡灌洗液中IgE,TGF-β1,TNF-α含量均高于正常组(P<0.05);喘平方组豚鼠肺泡灌洗液中IgE,TGF-β1,TNF-α含量均低于哮喘组(P<0.05)。结论:喘平方能够通过下调豚鼠肺泡灌洗液中IgE,TGF-β1,TNF-α含量,可降低气道高反应性、减轻气道炎症症状、控制或延缓气道纤维化进程。
Objective: To investigate the mechanism of ChuanPing Fang in preventing and treating bronchial asthma. Methods: Sixty albino guinea pigs were randomly divided into normal group, model group, ephedrine group (0.20 g.kg-1), Yangjinhua group (0.07 g.kg-1) 1) and dexamethasone group (7.5 × 10-4 mg · kg-1). Experimental guinea pigs were established by sensitization and inhalation of guinea pig’s ovalbumin. Guinea pigs in treatment group received daily ig Administration 1, 7 consecutive days, the normal group and model group given saline. The general situation of guinea pigs was observed. The changes of IgE, TGF-β1 and TNF-α in guinea pig lavage fluid were observed. Results: The levels of IgE, TGF-β1 and TNF-α in bronchoalveolar lavage fluid of guinea pigs were respectively as follows: IgE (68.25 ± 5.92) mg.L-1 and TGF-β1 (78.72 ± 10.92) TNF-α (388.02 ± 55.61) ng.L-1; The levels of IgE in the model group were (137.28 ± 14.38) mg.L-1, 172.39 ± 14.04 ng.L-1 and 752.76 ± 51.25, (76.35 ± 6.22) mg.L-1, TGF-β1 (115.76 ± 9.17) ng.L-1 and TNF-α (569.32 ± 39.7) ng.L- The levels of IgE, TGF-β1 and TNF-α in bronchoalveolar lavage fluid of guinea pigs in asthma group and asthma group were significantly higher than those in normal group (P <0.05). The levels of IgE, TGF-β1 and TNF-α in bronchoalveolar lavage fluid Content were lower than asthma group (P <0.05). Conclusion: ChuanPingFang can reduce the airway hyperresponsiveness, reduce the symptoms of airway inflammation and control or delay the process of airway fibrosis by down-regulating the content of IgE, TGF-β1 and TNF-α in the bronchoalveolar lavage fluid.