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To investigate the biological and immunological characteristics of the Nanjing local strains of HHV-7,fourstrains of herpesvirus were isolated from saliva specimens of one healthy individual and three childrensuffering from a kidney disease in Nanjing.The viruses were identified by transmission electron microscopy(EM),indirect immunofluorescence assay(IFA) with a specific monoclonal antibody;nested polymerase chainreaction,restriction mapping and DNA sequencing.The virus-infected cells showed the typical cytophathiceffect(CPE) under microscopy and could be detected by IFA with the human herpesvirus-7(HHV-7) specificantibody.Under EM,herpesvirus-like and virions capsids could be found in their cytoplasm or nucleoplasm.HHV-7 DNA fragments amplified from infected cells by nested PCR were confirmed by restriction mappingand DNA sequencing.Similarly to DC strain,an known HHV-7 strain used in the present study as the positivecontrol,the virus could be inactivated by ultraviolet irradiation for 10 min,heated at 45℃ for 30 min,pH<5or>9 at 4℃ for 2 h and ether or chloroform for 10 h.The virus induced the production of TNF-α,IL-10 andIL-12p70 while inhibited IFN-γ secretion,increased the percentage of CD2~+ cells while decreased that of CD4~+or CD45RA~+ cells.The results indicate that the viruses isolated in Nanjing are HHV-7,which has similarbiological characteristic to the known HHV-7 strain,DC.Infection with HHV-7 in vitro could affect immunefunction of lymphocytes by disturbing cytokine production and CD antigen expression.Cellular & MolecularImmunology.2004;1(5):367-372.
To investigate the biological and immunological characteristics of the Nanjing local strain of HHV-7, fourstrains of herpesvirus were isolated from saliva specimens of one healthy individual and three childrensuffering from a kidney disease in Nanjing. These viruses were identified by transmission electron microscopy (EM) , indirect immunofluorescence assay (IFA) with a specific monoclonal antibody; restriction mapping and DNA sequencing. virus-infected cells showed the typical cytophathiceffect (CPE) under microscopy and could be detected by IFA with the human herpesvirus-7 ( HHV-7) specific antigens. Under EM, herpesvirus-like and virions capsids could be found in their cytoplasm or nucleoplasm. HHV-7 DNA fragments amplified from infected cells by nested PCR were confirmed by restriction mapping and DNA sequencing. known HHV-7 strain used in the present study as the positive control, the virus could be inactivated by ultraviolet irradiation f or 10 min, heated at 45 ° C for 30 min, pH <5or> 9 at 4 ° C for 2 h and ether or chloroform for 10 h. The virus induced the production of TNF-α, IL-10 and IL-12 p70 while restricted IFN -γ secretion, increased the percentage of CD2 ~ + cells while decreased that of CD4 ~ + or CD45RA ~ + cells. The results indicates that the viruses isolated in Nanjing are HHV-7, which has similar biological characteristics to the known HHV-7 strain , DC. Infection with HHV-7 in vitro could affect immunefunction of lymphocytes by disturbing cytokine production and CD antigen expression. Cellular & Molecular Immunology. 2004; 1 (5): 367-372.