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探讨去乙酰化酶抑制剂丙戌酸钠（ＶＰＡ）与二甲双胍（ＭＥＴ）在前列腺癌 ＰＣ-３细胞中的抗肿瘤作用。方法 分别处理前列腺癌 ＰＣ-３细胞，分为对照组、ＶＰＡ组、ＭＥＴ组、ＶＰＡ＋ＭＥＴ组。随后利用 ＣＣＫ８检测肿瘤细胞的活性变化，Ｔｒａｎｓｗｅｌｌ技术检测肿瘤的侵袭转移能力，Ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ检测 ｐＡｋｔ、ｐＳｍａｄ３、ａＨ３、ａＨ４等的水平变化。结果 ＶＰＡ组与 ＭＥＴ组均可抑制肿瘤细胞的侵袭转移能力与肿瘤细胞活性，而 ＶＰＡ＋ＭＥＴ组对肿瘤细胞侵袭转移能力和肿瘤细胞活性的抑制较 ＶＰＡ组与 ＭＥＴ组更加显著。相比较对照组和 ＭＥＴ组中，ＶＰＡ＋ＭＥＴ组中 ｐＡｋｔ、ｐｍＴＯＲ和 ｐＳｍａｄ３蛋白水平明显降低，伴随 Ｈ３、Ｈ４乙酰化水平显著升高（Ｐ＜０．０５）。结论 ＶＰＡ与ＭＥＴ两种药物联合应用较单一用药具有更显著的优势。这种优势可能是通过调节转化生长因子（ＴＧＦ-β）与雷帕霉素靶蛋白（ｍＴＯＲ）两条通路活性及上调组蛋白 ａＨ３、ａＨ４而实现。
To investigate the antitumor effect of the deacetylase inhibitor sodium valproate (VPA) and metformin (MET) in prostate cancer PC-3 cells. Methods PC-3 cells were divided into control group, VPA group, MET group and VPA + MET group. Then the activity of tumor cells was detected by CCK8, the invasion and metastasis of tumor was detected by Transwell technique, and the levels of pAkt, pSmad3, aH3 and aH4 were detected by Western blotting. Results Both VPA group and MET group inhibited the invasion and metastasis of tumor cells and the activity of tumor cells. VPA + MET inhibited the invasion and metastasis of tumor cells and the activity of tumor cells more significantly than VPA group and MET group. The levels of pAkt, pmTOR and pSmad3 in VPA + MET group were significantly lower than those in control group and MET group, and the levels of acetylation of H3 and H4 were significantly increased (P <0.05). Conclusions VPA and MET combined with two drugs have more significant advantages than single drug. This advantage may be through the regulation of both TGF-β and rapamycin target protein (mTOR) pathway activity and upregulation of histone aH3, aH4 achieved.