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目的:通过观察补阳还五汤和桃红四物汤对兔自体皮片移植术后血管密度及组织VEGF表达量的变化,探讨益气活血中药对皮片移植成活的影响机制。方法:80只新西兰大耳白兔按随机数字表法分为4组,补阳还五汤组(a组),桃红四物汤组(b组),对照组(c组),空白组(d组),每组20只。a、b、c组均行背部皮片移植术造模,d组只剥离腹部皮片后缝合,不做移植。造模后用补阳还五汤和桃红四物汤分别干预a、b组两周,c、d组喂纯净水。于药物干预第7天和第14天分别取标本检测血管密度及运用双夹心ELISA法检测组织VEGF表达量。结果:两个不同时限a组和b组血管密度较c组和d组均明显提高,差异有统计学意义(P<0.05,P<0.01),a组和b组比较,差异无统计学意义(P>0.05);两个不同时限a组和b组组织VEGF表达量与c组和d组比较均明显升高,差异有统计学意义(P<0.05,P<0.01),a、b两组比较,a组表达量略高于b组,但差异无统计学意义(P>0.05)。结论:益气活血中药补阳还五汤及桃红四物汤能明显提高实验兔血管密度从而改善组织循环及血供,能显著提高移植皮片组织VEGF表达量,从而促进移植区域血管新生,提高新生血管数量。
OBJECTIVE: To observe the effect of Buyang Huanwu Decoction and Taohongsiwu Decoction on vascular density and tissue VEGF expression in autologous skin grafts after transplantation, and to explore the mechanism of Yiqi Huoxue traditional Chinese medicine on skin graft survival. Methods: Eighty New Zealand white rabbits were randomly divided into four groups according to random number table: Buyang Huanwu Decoction (group A), Taohongsiwutang Group (b), control group (c), blank group d group), 20 in each group. A, b, c group were performed dorsal skin graft surgery, d group only after stripping the abdominal skin flap, do not transplant. After modeling with Buyang Huanwu Decoction and Taohongsiwutang intervention a, b group two weeks, c, d group fed pure water. On the 7th day and the 14th day after the drug intervention, the specimens were taken to detect the vascular density and the expression of VEGF was detected by double sandwich ELISA. Results: The vascular densities in group a and group b at different time points were significantly higher than those in group c and d (P <0.05, P <0.01), but there was no significant difference between group a and group b (P> 0.05). The expression of VEGF in group a and group b at different time points was significantly higher than that in group c and group d, the difference was statistically significant (P <0.05, P <0.01) Compared with group b, the expression of group a was slightly higher than group b, but the difference was not statistically significant (P> 0.05). Conclusion: Buyang Huanwu Decoction and Taohongsiwutang can significantly improve the blood vessel density of experimental rabbits to improve the tissue circulation and blood supply, which can significantly increase the expression of VEGF in the transplanted skin tissue and promote angiogenesis in the transplanted area The number of new blood vessels.