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BACKGROUND: Fufang yimucao oral liquid has markedly effects on ameliorating circulation,restraining uterine constriction induced by oxytocin,alleviating dysmenorrhea,as a traditional medicine on promoting blood circulation by removing blood stasis,yimucao could ameliorate abnormal hemorrheological when hemorrhagic shock happens,enhance the hemoperfusion of organs and actively react on the result of hemorrhagic shock. OBJECTIVE: To investigate the abirritation of fufang yimucao oral liquid on pain model mice induced by hot board method and acetic acid twist body method and dysmenorrhea model mice induced by estradiol. DESIGN: Entirely randomly grouping and control experiment. SETTING: Pharmacological Laboratory,Henan College of Traditional Chinese Medicine. MATERIALS: A total of 200 female Kunming genus mice of grade 2 and weighing 18-21 g were collected. Fufang yimucao oral liquid,mainly consist of yimucao,danggui,chuanxiong,muxiang,and so on,was produced by Henan Joyline&Joysun Pharmaceutical Stock Co.,Ltd. (batch number: 050701); yimucao oral liquid was produced by Shangqiu Lvyuan Pharmaceutical Co.,Ltd. (batch number: 050108); estradiol slice by Shanghai Xinyi Kangjie Pharmaceutical Co.,Ltd. (batch number: 050301); YSL-6A intelligence hot plate instrument by Shandong Equipments Station of the Medical Science. METHODS: The experiment was carried out in the Animal Experiment Center of the Henan College of Traditional Chinese Medicine from August to November 2005. The high-,middle-and low-dosage fufang yimucao oral liquid in the experiment was 1,0.5 and 0.25 in volume fraction,respectively,and yimucao oral liquid was 0.5. ① Among 80 mice,60 mice were eligible in pain threshold tested by hot plate,and randomly dividing into 5 groups with 12 in each group. Mice in the high-,middle-and low-dose fufang yimucao oral liquid groups were perfused with 1 mL,0.5 mL and 0.25 mL/mL fufang yimucao,and mice in the yimucao group and saline group were perfused with the same volume yimucao oral liquid and saline,respectively,once a day for 3 successive days. Half an hour and one hour after administration for the last time,the range of pain was tested in hot plate and the increasing numerical value was counted. ② Another 60 mice were randomly divided into 5 groups. The grouping ways,numbers of animal and administration were as the same as those mentioned above. Forty minutes after administration for the last time,mice were given the celiac injecting with the fresh acetic acid,then observed and registered the delitescence of turned body and the times of turning in 10 minutes. ③ The rest of 60 mice were randomly divided into 6 groups with 10 in each group. The five groups were divided as the same as mentioned above,and the last group was the blank control group. Mice in the 5 former groups were given synestrin tablets CMC suspension with the dose of 2 mg/kg,0.1 g/L and 0.2 mL/10 g once a day for 12 successive days. Ten days later,mice were administrated as the same ways and dosage mentioned above. Mice in blank control group were perfused with the same volume of saline. Two days after modeling and 40 minutes after administration,mice were injected with oxytocin to observe the latent period and the frequency of twisting reaction in 10 minutes. MAIN OUTCOME MEASURES: Effect of fufang yimucao oral liquid on mice pain model induced by hot board method,twisting body response induced by acetic acid and mice dysmenorrhea model. RESULTS: ① Effect of fufang yimucao oral liquid on mice pain model induced by hot board: Thirty minutes after administration,the pain threshold of yimucao oral liquid group and high-,middle-and low-dose fufang yimucao oral liquid groups were respectively (25.42±2.10),(22.40±3.42),(25.24±2.51) and (19.80 ±2.00) s,which were markedly higher than saline group (17.98±1.68) s,P < 0.05-0.01. Sixty minutes after administration,the pain threshold of yimucao oral liquid group and high-,middle-and low-dose fufang yimucao oral liquid groups were respectively (27.42±2.17),(23.83±2.66),(27.64±2.64) and (21.51±2.41) s,which were markedly higher than saline group (17.8±1.75) s,P < 0.01. ② Effect of fufang yimucao oral liquid on twisting body response induced by celiac injecting acetic acid: The twisting body latent period of yimucao oral liquid group and high-,middle-and low-dose fufang yimucao oral liquid groups were respectively (2.43±0.19),(2.09±0.20),(2.60±0.14) and (1.85±0.35) s,which were markedly higher than saline group (1.45±0.22) s,P < 0.01. The twisting body times in 10 minutes of yimucao oral liquid group and high-,middle-and low-dose fufang yimucao oral liquid groups were respectively (14.8±4.0),(15.8± 3.5),(12.2±3.7) and (18.7±3.3) times,which were markedly lower than saline group (25.0±5.0) times,P < 0.01. ③ Effect of fufang yimucao oral liquid on mice dysmenorrhea model: After injecting estradiol,the twisting body latent period of yimucao oral liquid group and high-,middle-and low-dose fufang yimucao oral liquid groups were respectively (61.8±20.8),(105.8±29.8),(78.9±14.0) and (71.9±20.0) s,which were higher than the saline group (31.6±14.71) s,P < 0.01. The twisting body times in 10 minutes of yimucao oral liquid group and high-,middle-and low-dose fufang yimucao oral liquid groups were respectively (18.1±4.2),(9.5±2.8),(16.2±3.5) and (19.9±4.6) times,which were lower than the saline group (28.5±4.7) times,P < 0.01. CONCLUSION: Fufang yimucao oral liquid has a good effect on abirritation,condignly as yimucao oral liquid,besides it does not have obvious dependent effect.
BACKGROUND: Fufang yimucao oral liquid has markedly effects on ameliorating shock, restraining uterine constriction induced by oxytocin, alleviating dysmenorrhea, as a traditional medicine on promoting blood circulation organs and actively react on the result of hemorrhagic shock. OBJECTIVE: To investigate the abirritation of fufang yimucao oral liquid on pain model mice induced by hot board method and acetic acid twist body method and dysmenorrhea model mice induced by estradiol. DESIGN: Entirely randomly grouping MATERIALS: A total of 200 female Kunming genus mice of grade 2 and weighing 18-21 g were collected. Fufang yimucao oral liquid, mainly consisting of yimucao, danggui, chuanxiong, muxiang, and so on, was produced by Henan Joyline & Joysun Pharma (batch number: 050701); yimucao oral liquid was produced by Shangqiu Lvyuan Pharmaceutical Co., Ltd. (batch number: 050108); estradiol slice by Shanghai Xinyi Kangjie Pharmaceutical Co., Ltd. : 050301); YSL-6A intelligence hot plate instrument by Shandong Equipments Station of the Medical Science. METHODS: The experiment was carried out in the Animal Experiment Center of the Henan College of Traditional Chinese Medicine from August to November 2005. The high- middle-and low-dosage fufang yimucao oral liquid in the experiment was 1, 0.5 and 0.25 in volume fraction, respectively, and yimucao oral liquid was 0.5. ① Among 80 mice, 60 mice were eligible in pain threshold tested by hot plate, and randomly divided into 5 groups with 12 in each group. Mice in the high-, middle-and low-dose fufang yimucao oral liquid groups were perfused with 1 mL, 0.5 mL and 0.25 mL / mL fufang yimucao, and mice in the yimucao group and saline group were perfused with the same volum ehalf an hour and one hour after administration for the last time, the range of pain was tested in hot plate and the increasing numerical value was counted. ② Another 60 mice were randomly divided into 5 groups. The grouping ways, numbers of animal and administration were the same as those mentioned above. Forty minutes after administration for the last time, mice were given the ciaciac injecting with the fresh acetic acid, then observed and registered the delitescence of turned body and the times of turning in 10 minutes. ③ The rest of 60 mice were randomly divided into 6 groups with 10 in each group. The five groups were divided as the same as mentioned above, and the last group was the blank control group. Mice in the 5 former groups were given synestrin tablets CMC suspension with the dose of 2 mg / kg, 0.1 g / L and 0.2 mL / 10 g once a day for 12 successive days. Ten days later, the mice were administered as the s ame ways and dosage mentioned above. Mice in blank control group were perfused with the same volume of saline. Two days after modeling and 40 minutes after administration, mice were injected with oxytocin to observe the latent period and the frequency of twisting reaction in 10 minutes . MAIN OUTCOME MEASURES: Effect of fufang yimucao oral liquid on mice pain model induced by hot board method, twisting body response induced by acetic acid and mice dysmenorrhea model. RESULTS: ① Effect of fufang yimucao oral liquid on mice pain model induced by hot board : Thirty minutes after administration, the pain threshold of yimucao oral liquid group and high-, middle-and low-dose fufang yimucao oral liquid groups were respectively (25.42 ± 2.10), (22.40 ± 3.42), (25.24 ± 2.51) and 19.80 ± 2.00) s, which were markedly higher than saline group (17.98 ± 1.68) s, P <0.05-0.01 Sixty minutes after administration, the pain threshold of yimucao oral liquid group and high-, middle-and low-dose fufang yimucao oral li quid groups(27.42 ± 2.17), (23.83 ± 2.66), (27.64 ± 2.64) and (21.51 ± 2.41) s, which were markedly higher than saline group (17.8 ± 1.75) s, P <0.01. ② Effect of fufang yimucao oral liquid on twisting body response induced by ciaciac injecting acetic acid: The twisting body latent period of yimucao oral liquid group and high-, middle-and low-dose fufang yimucao oral liquid groups were respectively (2.43 ± 0.19), (2.09 ± 0.20 (2.60 ± 0.14) and (1.85 ± 0.35) s, which were markedly higher than saline group (1.45 ± 0.22) s, P <0.01. The twisting body times in 10 minutes of yimucao oral liquid group and high-, middle -and low-dose fufang yimucao oral liquid groups were respectively (14.8 ± 4.0), (15.8 ± 3.5), (12.2 ± 3.7) and (18.7 ± 3.3) times, which were markedly lower than saline group (25.0 ± 5.0) times , P <0.01. ③ Effect of fufang yimucao oral liquid on mice dysmenorrhea model: After injecting estradiol, the twisting body latent period of yimucao oral liquid group and high-, middle-and low-dose fufan g yimucao oral liquid groups were (61.8 ± 20.8), (105.8 ± 29.8), (78.9 ± 14.0) and (71.9 ± 20.0) s, respectively, which were higher than the saline group (31.6 ± 14.71) s, P <0.01. The twisting body times in 10 minutes of yimucao oral liquid group and high-, middle-and low-dose fufang yimucao oral liquid groups were respectively (18.1 ± 4.2), (9.5 ± 2.8), (16.2 ± 3.5) and (19.9 ± 4.6) times, which were lower than the saline group (28.5 ± 4.7) times, P <0.01 CONCLUSION: Fufang yimucao oral liquid has a good effect on abirritation, condignly as yimucao oral liquid, besides it does not have obvious dependent effect.