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采用遮荫网设置4个太阳辐射通量梯度(20%、40%、60%、100%),对小麦生长季麦田土壤进行太阳辐射减弱的试验,用BIOLOG生态测试板(ECO MicroPlate),采用孔平均颜色变化率法(average well-color development,AWCD)测定不同太阳辐射通量处理下的冬小麦根际土壤微生物利用单一碳源的能力,计算微生物群落多样性指数,对不同碳源的利用率进行主成分分析,重复2年的试验,研究太阳辐射减弱对麦田土壤微生物多样性的影响。结果表明:1)太阳辐射减弱,土壤微生物对单一碳源的利用速度降低,碳源分解的总量降低;2)土壤微生物优势种群、群落丰富度和个体数量在群落中的分布状况未受到太阳辐射减弱的直接影响;3)微生物对大多数碳源的代谢能力减弱,糖类代谢受辐射减弱的影响最大,变异度最高。结果说明,小麦根际土壤微生物碳代谢受地表太阳辐射减弱的影响,这为了解大气环境变化对土壤微生态的影响提供了参考。
Four sun radiation flux gradients (20%, 40%, 60%, 100%) were set up using shading net to test the solar radiation weakened in wheat field growing season wheat field. BIOLOG EcoPlate was used, Average well-color development (AWCD) was used to determine the ability of single rhizospheric soil microbes to use single carbon source under different solar radiation fluxes to calculate the microbial community diversity index, and the utilization of different carbon sources Principal component analysis was conducted and the experiment was repeated for 2 years to study the effect of weaker solar radiation on soil microbial diversity in wheat field. The results showed that: 1) the solar radiation decreased, the utilization rate of single carbon source by soil microorganism decreased, and the total amount of carbon source decomposition decreased; 2) the distribution of dominant species, community richness and number of individuals in the community was not affected by the sun The direct effect of weakened radiation; 3) the metabolic capacity of microorganisms to most of the carbon sources weakened, and the carbohydrate metabolism was the most affected by the weakened radiation with the highest variability. The results showed that the microbial metabolism of rhizosphere soil was affected by the decrease of surface solar radiation, which provided a reference for understanding the influence of atmospheric environment on soil micro-ecology.