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目的采用HPLC波长切换法,建立同时测定补肾活血颗粒中松脂醇二葡萄糖苷、苦杏仁苷、桂皮醛3成分含量的分析方法。方法采用Zorbax Extend-C18柱(4.6 mm×150 mm,5μm);以乙腈-水为流动相梯度洗脱,松脂醇二葡萄糖苷、苦杏仁苷、桂皮醛的检测波长分别为227,210,290 nm;进行样品前处理工艺和方法学考察。结果松脂醇二葡萄糖苷、苦杏仁苷和桂皮醛均得到很好分离,浓度分别在0.426 0~2.88 6μg·m L?1,7.455~86.60μg·m L?1和0.440 6~13.22μg·m L?1内线性关系良好(r≥0.999);平均加样回收率分别为98.3%(RSD=1.6%),101.4%(RSD=1.1%)和100.0%(RSD=1.2%);精密度试验峰面积RSD均≤1.4%。结论该方法预处理简单,方法灵敏、准确、重复性好,可用于补肾活血颗粒及相关制剂的质量控制。
OBJECTIVE To establish a method for the simultaneous determination of Pinosin diglucoside, amygdalin and cinnamaldehyde in Bushen Huoxue granule by HPLC wavelength switching method. Methods The Zorbax Extend-C18 column (4.6 mm × 150 mm, 5 μm) was used as the mobile phase. The detection wavelength was 227, 210 and 290 nm for pinoresinol diglucoside, amygdalin and cinnamaldehyde with acetonitrile-water as mobile phase. Pretreatment process and methodological study. Results Pinoresinol diglucoside, amygdalin and cinnamaldehyde were well separated at concentrations ranging from 0.426 0 to 2.88 6 μg · mL -1, 1,7.455 ~ 86.60 μg · mL -1 and 0.440 6 ~ 13.22 μg · m -1 The average recovery was 98.3% (RSD = 1.6%), 101.4% (RSD = 1.1%) and 100.0% (RSD = 1.2%), respectively. The precision test Peak area RSD ≤ 1.4%. Conclusion The method is simple, sensitive, accurate and reproducible. It can be used for quality control of Bushen Huoxue Granules and related preparations.