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目的:观察模拟失重对大鼠胸主动脉氧化应激水平的影响,探讨其可能机制。方法:采用3周尾部悬吊大鼠模型模拟失重状态,通过DHE荧光探针技术观察大鼠动脉血管超氧阴离子水平变化,通过比色法测定大鼠动脉血管丙二醛(MDA)含量,通过蛋白印记技术观察悬吊(SUS)大鼠和正常对照(CON)大鼠动脉血管NOX4、p22phox的表达变化。结果:尾部悬吊3周后,SUS组大鼠胸主动脉超氧阴离子水平较CON组明显增高,SUS组(0.849±0.023 nmol/mg protein)大鼠MDA含量较CON组(0.575±0.054nmol/mg protein)明显增加;SUS组大鼠胸主动脉的p22phox及NOX4蛋白表达均较CON组明显增强。结论:模拟失重3周可使大鼠胸主动脉氧化应激水平明显增高,p22phox及NOX4蛋白表达明显增多,结果提示,尾部悬吊模拟失重状态下氧化应激水平增高可能与NADPH氧化酶表达增高有关。
Objective: To observe the effect of simulated weightlessness on oxidative stress in rat thoracic aorta and to explore its possible mechanism. Methods: The tail suspension model was used to simulate the state of weightlessness in rats after 3 weeks. The changes of the superoxide anion in arterial blood were observed by DHE fluorescence probe. The content of malondialdehyde (MDA) Western blotting was used to observe the expression of NOX4 and p22phox in the artery of SUS rats and normal control rats. RESULTS: After 3 weeks of tail suspension, the level of superoxide anion in thoracic aorta in SUS group was significantly higher than that in CON group. The content of MDA in SUS group (0.849 ± 0.023 nmol / mg protein) was significantly higher than that in CON group (0.575 ± 0.054 nmol / mg protein). The expressions of p22phox and NOX4 protein in thoracic aorta of SUS group were significantly higher than that of CON group. CONCLUSION: Three weeks after simulated weightlessness, the oxidative stress in rat thoracic aorta was significantly increased and the expressions of p22phox and NOX4 protein were significantly increased. The results suggest that the increased oxidative stress may be related to the increased expression of NADPH oxidase in tail suspension simulated weightlessness related.