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山东近海褐牙鲆自然群体活样本共 79尾 ,分别于 1 996年 5月、1 997年 1月和 1 998年 4月采自青岛近海 ;养殖群体活样本 5 2尾于 1 997年 1 2月采自山东荣成寻山养鱼场。采用水平淀粉胶和垂直聚丙烯酰胺凝胶电泳方法及 3种缓冲系统 (TC、EBT和TG)分别对自然和养殖群体的 1 5种同工酶进行生化遗传分析。结果表明 ,山东近海牙鲆自然群体的多态基因座位比例 ( 31 .0 % )和群体平均杂合度 ( 0 .0 80 2 )都明显高于养殖群体 ( 2 4 .1 % ,0 .0 788) ;在自然群体的 9个多态基因座位、养殖群体的 7个多态基因座位中 ,除了Cat(P <0 .0 5 )和Idhp - 1(P <0 .0 5 ,养殖群体中 )有显著差异、Ldh -C(P <0 .0 1 )完全偏离Hardy -Weinberg定律外 ,其余多态座位基因频率均符合Hardy-Weinberg遗传平衡定律
A total of 79 live samples of native Japanese flounder (Paralichthys olivaceus) were collected from the coastal waters of Qingdao in May 1996, January 1997 and April 1998, respectively. A total of 52 2 live samples from cultured populations were collected in 1997 Month mining from Shandong Rongcheng Xun mountain fish farm. Fifteen isozymes from natural and cultured populations were biochemically analyzed by horizontal starch gel and vertical polyacrylamide gel electrophoresis and three buffer systems (TC, EBT and TG). The results showed that the proportion of polymorphic loci (31.0%) and population average heterozygosity (0.0802) in native populations of Japanese flounder were significantly higher than those of cultured population (24.1%, 0.0788 ); Among the 9 polymorphic loci in natural populations, except for Cat (P <0.05) and Idhp - 1 (P <0.05), among the seven polymorphic loci in cultured population, (P <0.01) completely deviated from the Hardy-Weinberg law, the rest of the polymorphic loci frequency in line with the Hardy-Weinberg law of genetic balance