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目的 :探讨百草枯对黑质细胞线粒体呼吸链的影响和导致黑质细胞损害的可能机制。方法 :测定通过口服途径亚慢性暴露于百草枯、MPTP或等量生理盐水的小鼠脑内线粒体呼吸链中复合酶Ⅰ、Ⅱ、Ⅳ及柠檬酸合成酶的活性。结果 :百草枯和MPTP干预组小鼠黑质部及纹状体线粒体呼吸链中复合酶Ⅰ的活性显著降低 ,额叶和小脑中该酶的活性无变化。各组小鼠黑质部、纹状体、额叶及小脑中复合酶Ⅱ、Ⅳ及柠檬酸合成酶的活性均无显著差异。结论 :百草枯透过血脑屏障后选择性地导致黑质部和纹状体中线粒体功能障碍。
OBJECTIVE: To investigate the effect of paraquat on the respiratory chain of mitochondria of substantia nigra cells and the possible mechanism of the damage of nigra cells. Methods: The activity of complex enzymes I, II, IV and citrate synthase in mitochondrial respiratory chain of mice brain sub-chronicly exposed to paraquat, MPTP or normal saline by oral route was determined. Results: The activity of compound enzyme Ⅰ in the mitochondrial respiratory chain of the substantia nigra and striatum was significantly decreased in the paraquat and MPTP-treated groups, while the activity of the enzyme in the frontal lobe and cerebellum was unchanged. There was no significant difference in the activities of complex enzymes Ⅱ, Ⅳ and citrate synthase in the substantia nigra, striatum, frontal lobe and cerebellum of mice in each group. CONCLUSION: Paraquat selectively causes mitochondrial dysfunction in the substantia nigra and striatum after passing through the blood-brain barrier.