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To identify the water stress induced genes of upland rice cultivar IRAT109,which is resistant to drought,a subtractivecDNA library was developed from polyethylene glycol-(PEG)treated and non-treated seedlings by suppression subtractivehybridization,from which 2112 recombinant colonies were obtained.Eight hundred clones were selected randomly forsequencing analysis,and 384 unique expressed sequence tags(ESTs)were obtained.They were found to be involved indiverse biological processes,such as metabolism,transcription,signal transduction,protein synthesis and others.Notablya number of known functional genes in drought tolerance,including genes related to biosynthesis of osmoprotectants,defense against active oxygen,removal of toxic compounds,recovery of proteins and reinforcement of cell wall werealso found in the study.Several genes related to deleterious responses were upregulated by PEG stress.The differentialexpression patterns of 11 SSH-derived ESTs were confirmed by real-time polymerase chain reaction.
To identify the water stress induced genes of upland rice cultivar IRAT 109, which is resistant to drought, a subtractive cDNA library was developed from polyethylene glycol- (PEG) treated and non-treated seedlings by suppression subtractive hybridization, from which 2112 colonies were obtained. Light hundred clones were selected randomly forsequencing analysis, and 384 unique expressed sequence tags (ESTs) were obtained. They were found to be involved indiverse biological processes, such as metabolism, transcription, signal transduction, protein synthesis and others. Notablya number of known functional genes in drought tolerance, including genes related to biosynthesis of osmoprotectants, defense against active oxygen, removal of toxic compounds, recovery of proteins and reinforcement of cell wall were found in the study. Selerral genes related to deleterious responses were upregulated by PEG stress. differentially expression patterns of 11 SSH-derived ESTs were confirmed by real-time polymer ase chain reaction.