沙坡头地区根瘤菌DNA同源性及16SrDNA全序列

• 作 者: 李颖 白雨 陈文新
• 机 构: 中国农业大学生物学院
• 出 处: 微生物学报
• 发表日期: 1999年02期
• 关键词: DNA同源性 沙坡头 数值分类 多位点酶电泳 互接种族 全序列分析 豆科植物 系统发育树 土壤改良 山羊豆

数值分类和多位点酶电泳分析表明，分离自宁夏沙坡头地区的１２株根瘤菌构成一个独立的表观群。对这一菌群进行了ＤＮＡ同源性和群内中心菌株１６ＳｒＤＮＡ全序列分析。１２个菌株的Ｇ＋Ｃｍｏｌ％在５６４～６２２范围内；群内ＤＮＡ同源性为７２３％～９３５％，大于７０％，属种内水平；中心株Ｎ２２０的１６ＳｒＤＮＡ全序列与参比菌株的序列比较，从模拟系统发育树看出，它与三株土壤杆菌、三株根瘤菌的１６ＳｒＤＮＡ序列同源性在９４８％～９９２％的相似性水平上构成一个分支，看来沙坡头地区这群根瘤菌是一个独立的新种群。

Numerical classification and multi-site enzyme electrophoresis analysis showed that 12 rhizobia isolated from Shapotou area in Ningxia constitute an independent apparent population. DNA sequence homology and full-length 16S rDNA sequence analysis of this group were carried out. The G + C mol% of 12 strains ranged from 564 to 622. The DNA homology in the population was 723% -935% and greater than 70%, belonging to the intraspecific level. The 16S rDNA sequence of central strain N220 Compared with the reference strains, the 16SrDNA sequence homology with three strains of Agrobacterium tumefaciens and three strains of Rhizobium showed a similarity of 948% -992% from the simulated phylogenetic tree It seems that this group of rhizobia in Shapotou is an independent new population.