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目的:本研究探讨新型维甲酸衍生物4-氨基-2-三氟甲基苯基依曲替酸酯(4-amino-2-trifluoromethyl-phenyl acitretinate,ATPA)对白血病K562细胞体外增殖和分化的作用。方法:用不同浓度的ATPA作用白血病K562细胞后,在体外通过MTT法检测和绘制细胞生长曲线来分析细胞增殖;瑞特染色法观察细胞形态学改变;氯化硝基四氮唑蓝(nitroblue tetrazolium chloride,NBT)还原实验分析细胞的分化指标;采用FCM法检测细胞表面分化抗原及细胞周期的变化。结果:ATPA呈浓度依赖性抑制K562细胞增殖,明显的抑制作用从药物作用48h后开始出现,在72h后作用更明显。倒置显微镜下观察发现ATPA作用后K562细胞形态趋向成熟,NBT阳性细胞率增加;G0/G1期细胞表达量增加,S期细胞表达量减少,呈G0/G1期阻滞;细胞表面分化抗原CD71表达减少。结论:ATPA对白血病K562细胞具有抑制增殖和一定的诱导分化作用。
AIM: To investigate the in vitro proliferation and differentiation of leukemic K562 cells induced by 4-amino-2-trifluoromethyl-phenyl acitretinate (ATPA), a novel retinoid. effect. Methods: After leukemia K562 cells were treated with different concentrations of ATP, cell proliferation was detected by MTT assay in vitro and cell growth curve was drawn. Cell morphology was observed by Reiter staining. Nitroblue tetrazolium chloride, NBT) reduction experiments were used to analyze the differentiation of cells; FCM was used to detect the cell surface differentiation antigen and cell cycle. Results: ATPA inhibited the proliferation of K562 cells in a concentration-dependent manner. The obvious inhibitory effect began to appear after 48 hours of drug treatment and was more pronounced after 72 hours. Under inverted microscope, the morphology of K562 cells matured and the percentage of NBT positive cells increased after treated with ATPA. The expression of cells in G0 / G1 phase increased and the expression of S0 phase cells decreased in G0 / G1 phase. The expression of cell surface differentiation antigen CD71 cut back. Conclusion: ATPA can inhibit proliferation and induce differentiation of leukemia K562 cells.