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目的:研究中药柴芍承气汤对大鼠肠缺血再灌注(I/R)保护作用和机理。方法:30只大鼠分为3组,假手术对照组、I/R模型组和中药治疗组各10只。通过免疫组织化学染色方法和RT-PCR法检测大鼠肠组织CysLTR1蛋白和mRNA表达水平,并应用光学显微镜观察小肠病理改变。结果:1与对照组比较,中药治疗组大鼠肠道组织的病理改变均明显减轻。2肠I/R时肠组织CysLTR1蛋白和mRNA表达水平升高;应用中药后,肠组织CysLTR1蛋白和mRNA表达水平均减低。结论:柴芍承气汤能减轻肠I/R肠道组织的病理损害,并能抑制大鼠肠黏膜抑制肠组织CysLTR1的表达,CysLTR1的表达可能是柴芍承气汤保护肠黏膜损伤的靶点。
Objective: To study the protective effect and mechanism of Chai Shao Cheng Qi Decoction on rat intestinal ischemia reperfusion (I / R). Methods: Thirty rats were divided into three groups: sham operation control group, I / R model group and TCM treatment group. The expression of CysLTR1 protein and mRNA in intestinal tissue were detected by immunohistochemical staining and RT-PCR. Pathological changes of small intestine were observed with light microscope. Results: 1 Compared with the control group, the pathological changes of intestinal tissue in the TCM-treated group were significantly reduced. The expression of CysLTR1 protein and mRNA in the intestine of intestine was increased when the intestinal I / R was administered. The protein and mRNA expression of CysLTR1 in the intestine decreased after the application of traditional Chinese medicine. Conclusion: Chai Sha Cheng Cheng Qi Decoction can reduce intestinal I / R pathological damage, and can inhibit the intestinal mucosa of rats inhibit the expression of CysLTR1, CysLTR1 expression may be Chaishao Chengqi Decoction to protect intestinal mucosal injury target point.