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目的:探讨病毒性肝炎患者细胞凋亡情况及其临床意义。方法:应用DNA缺口末端标记技术检测了28例各型病毒性肝炎肝组织细胞凋亡密度和分布,以8例正常肝组织为对照。结果:DNA缺口末端标记技术能较好地检测肝细胞凋亡,阳性细胞多单个散在于肝小叶内,以门管区周围较多见。比较各型肝炎的凋亡细胞密度发现,重型肝炎的凋亡细胞密度高于慢性肝炎和肝硬变(P均<0.01);慢性肝灸和肝硬变的凋亡细胞密度高于急性肝炎(P<0.01)。结论:细胞凋亡可能与病毒性肝炎的慢性化有关;也可能是重型肝炎的发病机制之一。
Objective: To investigate the apoptosis of patients with viral hepatitis and its clinical significance. Methods: Apoptotic density and distribution of hepatic cells in 28 cases of various types of viral hepatitis were detected by DNA nick end labeling. Eight normal liver tissues were used as controls. Results: The DNA nick end labeling technique could detect the apoptosis of hepatocytes well, and the number of positive cells scattered in the hepatic lobule was more than that of the portal area. Compared with the density of apoptotic cells in various types of hepatitis, the density of apoptotic cells in severe hepatitis was higher than those in chronic hepatitis and cirrhosis (all P <0.01). The density of apoptotic cells in chronic liver moxibustion and cirrhosis was higher than that in acute hepatitis P <0.01). Conclusion: Apoptosis may be related to the chronicity of viral hepatitis and may also be one of the pathogenesis of severe hepatitis.