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目的观察六氧化四砷(As_4O_6)对小鼠胃癌生长和转移的抑制作用。方法完整组织块SCID鼠胃壁原位种植,建立胃癌转移模型。将裸鼠40只随机分为4组,每组10只。移植后第7天开始,分别给予生理盐水(对照组)、5-氟尿嘧啶(5-Fu组)、As_4O_6组、5-Fu和As_4O_6联合应用(5-Fu+As_4O_6组),每日1次,共6周。种植后第8周末处死动物,原位肿瘤切除称重,计算抑瘤率、测定肿瘤微血管密度(MVD)和细胞凋亡指数(AI),并检查转移情况,计算转移抑制率。结果与对照组比较,5-Fu组、As_4O_6组和5-Fu+As_4O_6组的抑瘤率分别为46.3%、52.2%和67.9%(均P<0.05),MVD分别为(12.2±5.2)、(7.1±3.5)(P<0.05)和(5.6±3.3)(P<0.05),AI分别为(6.82±4.27)、(10.51±6.19)(P<0.05)和(14.66±7.54)(P<0.05),肝转移抑制率分别为25%、55%(P<0.05)和70%(P<0.05),腹膜转移抑制率分别为22.9%、61.4%(P<0.05)和87.1%(P<0.05)。As_4O_6组和5-Fu+As_4O_6组胃癌生长和转移受到明显抑制,以5-Fu和As_4O_6联用组效果最明显。结论As_4O_6通过抑制血管生成对胃癌生长和转移有明显抑制作用,与5-Fu联用可起协同抑制作用。
Objective To observe the inhibitory effect of arsenic tetraoxide (As_4O_6) on the growth and metastasis of gastric cancer in mice. Methods The gastric tissue of SCID mice was implanted in situ and the gastric cancer metastasis model was established. 40 nude mice were randomly divided into 4 groups of 10. The rats in control group, 5-Fu group, As_4O_6 group, 5-Fu group and As_4O_6 group were treated with 5-Fu + As_4O_6 once daily on the 7th day after transplantation, A total of 6 weeks. At the end of the 8th week after implantation, the animals were sacrificed and resected and weighed in situ. The tumor inhibition rate was calculated. MVD and AI were measured. Metastasis was evaluated and the rate of metastasis was calculated. Results Compared with the control group, the tumor inhibition rates of 5-Fu, As 4 O 6 and 5-Fu + As 4 O 6 groups were 46.3%, 52.2% and 67.9% (all P <0.05) (7.1 ± 3.5) and (5.6 ± 3.3), respectively (P <0.05) and AI were 6.82 ± 4.27, 10.51 ± 6.19 and 14.66 ± 7.54, respectively (P < 0.05). The inhibition rate of hepatic metastasis was 25%, 55% (P <0.05) and 70% (P <0.05) respectively. The inhibition rates of peritoneal metastasis were 22.9%, 61.4% 0.05). The growth and metastasis of gastric cancer in As 4 O 6 and 5-Fu + As 4 O 6 groups were significantly inhibited, and the combination of 5-Fu and As 4 O 6 was the most effective. Conclusion As4O6 can significantly inhibit the growth and metastasis of gastric carcinoma by inhibiting angiogenesis, and synergistic inhibition with As-4O6 can be obtained when combined with 5-Fu.