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目的:初步探索HOXA10的辅因子(Meis1)与子宫内膜异位症(EMs)患者不孕和发病的相关性。方法:采用免疫组织化学方法进行组织学定位并进行半定量分析,采用Westernblot-ting方法在蛋白水平上进行定量分析,检测EMs不孕患者异位和在位子宫内膜中Meis1的表达水平。结果:免疫组织化学结果显示,Meis1在异位内膜和在位内膜中仅有弱表达,而在正常同期内膜中呈较明显的阳性表达,差异显著(P<0.01);比较Meis1在分泌中期在位内膜与正常内膜中的表达,差异亦有显著性(P<0.01)。Westernblotting显示,Meis1在分泌中期在位内膜中仅有弱表达,而在同期正常内膜中呈高表达(P<0.001)。结论:Meis1在EMs患者分泌中期在位内膜中低表达,可能是影响子宫内膜容受性的建立,从而影响胚胎着床导致不孕的重要因素之一。同时,Meis1在异位内膜中的低表达说明异位内膜可能对体内雌、孕激素正常调控具有抵抗性,异位内膜细胞具备了对抗凋亡的能力,提示Meis1可能参与了EMs的发生与发展。
Objective: To explore the correlation between HOXA10 cofactor (Meis1) and infertility and pathogenesis in patients with endometriosis (EMs). Methods: Immunohistochemistry was used for histological localization and semi-quantitative analysis. Western blotting was used to quantify protein levels and detect the expression of Meis1 in ectopic and eutopic endometrium of infertile women with EMs. Results: The results of immunohistochemistry showed that Meis1 was only weakly expressed in the ectopic endometrium and eutopic endometrium, but significantly higher in the normal endometrium (P <0.01). Compared with Meis1 There was also a significant difference in the secretory expression between eutopic endometrium and normal endometrium (P <0.01). Western blotting showed that Meis1 was weakly expressed in the eutopic endometrium during the secretory phase and was highly expressed in the normal endometrium at the same period (P <0.001). CONCLUSION: Meis1 is lowly expressed in the eutopic endometrium during the mid-term secretion of EMs, which may be one of the important factors that affect the establishment of endometrial receptivity and thus affect the embryo implantation. Meanwhile, the low expression of Meis1 in ectopic endometrium indicates that ectopic endometrium may be resistant to the normal regulation of estrogen and progesterone in vivo, and the ectopic endometrial cells possess the ability of anti-apoptosis, suggesting that Meis1 may be involved in EMs Occurrence and development.