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目的:建立一种以模拟阻塞性睡眠呼吸暂停低通气综合征的慢性间歇低氧大鼠模型为基础、探讨在体舌下神经电信号活动的实验平台。方法:慢性间歇低氧大鼠模型由气源(氮气及空气)、电磁阀控制系统、间歇低氧动物仓、以及实验动物(大鼠)组成,按照实验要求对大鼠交替给予氮气和空气,每天8h,连续处理7周后,分离舌下神经,应用多通道生物信号采集与处理系统对舌下神经电信号进行处理与分析。结果:预实验结果显示,慢性间歇低氧大鼠模型能够模拟人体阻塞性睡眠呼吸暂停低通气综合征的病理生理特征,所记录的电信号稳定、可靠。结论:慢性间歇低氧大鼠模型简单、易行、实用、重复性好,记录的舌下神经电信号稳定、可靠,达到实验设计要求。
OBJECTIVE: To establish a kind of chronic intermittent hypoxic rat model that simulates obstructive sleep apnea-hypopnea syndrome (Hypopnea Syndrome), and to explore the experimental platform for the activity of somatic electrical signals in the body. Methods: The chronic intermittent hypoxic rat model consisted of gas source (nitrogen and air), solenoid valve control system, intermittent hypoxia tank, and experimental animals (rats). Nitrogen and air were alternately given to rats according to the experimental requirements. The hypoglossal nerve was isolated after 8 weeks of continuous treatment for 7 weeks and the hypoglossal nerve electrical signals were processed and analyzed using a multi-channel biological signal acquisition and processing system. Results: The preliminary experimental results showed that the chronic intermittent hypoxic rat model can simulate the pathophysiological characteristics of human obstructive sleep apnea-hypopnea syndrome, and the recorded electrical signals are stable and reliable. Conclusion: The chronic intermittent hypoxic rat model is simple, easy, practical and reproducible. The recorded hypoglossal nerve signals are stable and reliable, and meet the experimental design requirements.