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研究了用９９ｍＴｃ直接标记抗体单价片段Ｆａｂ’的方法与条件．ＩｇＧ型多抗经无花果蛋白酶消化后得到Ｆ（ａｂ＇）２双价片段，再经２－巯基乙胺还原为Ｆａｂ＇单价片段，与９９ｍＴｃ及亚锡葡萄糖酸钠药盒溶液混合保温，即可得到９９ｍＴｃ－Ｆａｂ’标记物，标记率为８５％－９５％。这标记物在小鼠血中的清除速率明显高于完整抗体标记物９９ｍＴｃ－ＩｇＧ．
The method and conditions for directly labeling the antibody monovalent fragment Fab ’with 99mTc were studied. IgG polyclonal antibody is digested by protease to obtain F (ab ’) 2 bivalent fragment, and then reduced to Fab’ monovalent fragment by 2-mercaptoethylamine, mixed with 99mTc and sodium stannous gluconate kit for incubation, then The 99mTc-Fab ’tag was obtained with a labeling rate of 85% -95%. The rate of clearance of this marker in mouse blood was significantly higher than that of the intact antibody marker 99mTc-IgG.