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本研究旨在探讨与动物空间辨别学习能力相关的神经振荡电活动及其改变。运用Y型迷宫电击回避训练方法,筛选出与空间认知能力相关的快回避反应组和普通回避反应组大鼠,无线遥测两组动物在电击回避实验前、后海马CA3区实时局部场电位(local field potential,LFP),分析与空间辨别和学习能力相关的神经振荡成分变化。结果显示,与普通回避反应组大鼠比较,电击回避训练前快回避反应组大鼠左侧海马CA3区LFP成分无显著差异,但电击回避训练后,右侧海马CA3区0~10 Hz和30~40 Hz电节律百分比显著增加(P<0.01或P<0.05);快速傅里叶变换显示,0~10 Hz频段百分比增加主要发生在θ波(3~7 Hz)频段,而30~40 Hz频段改变相当于γ1频段。进一步将两组大鼠训练前后的右侧CA3区神经振荡进行自身比较,结果显示训练后快回避反应组大鼠仅出现β波、β2(20~30 Hz)和γ1节律百分比增加,θ波节律百分比在训练前后无明显变化,而普通回避反应组大鼠训练前后比较显示,训练后右侧CA3区θ波节律百分比和大幅波(强度:+2.5~-2.5 db)显著减少(P<0.01)。本研究结果显示,快回避反应组大鼠电击回避训练后,右侧海马CA3区β2和γ1节律百分比增加,θ波节律百分比保持较高水平,这些改变可能与其较强的空间认知能力有关。
The aim of this study was to investigate the electrical activity of neural oscillations and their alterations related to the spatial discernment ability of animals. Y-type maze shock avoidance training method was used to screen out the spatial cognition-related fast-avoidance response group and the general avoidance response group. The wireless telemetry in both groups before and after the shock avoidance experiment, the real-time local field potential in the hippocampal CA3 area local field potential (LFP)) to analyze the changes in the components of the neural oscillations associated with spatial discernment and learning ability. The results showed that there was no significant difference in LFP composition in the left hippocampal CA3 region of the traumatic avoidance response group before shock avoidance training compared with the normal avoidance response group. However, after shock avoidance training, the right hippocampal CA3 0-10 Hz and 30 The percentage of rhythms of ~ 40 Hz increased significantly (P <0.01 or P <0.05). The fast Fourier transform showed that the percentage increase of 0 ~ 10 Hz mainly occurred in the frequency band of 3 ~ 7 Hz, while the frequency of 30 ~ 40 Hz Band changes equivalent to γ1 band. The results showed that only the β wave, β2 (20-30 Hz) and γ1 rhythmic percentage increased, while θ rhythm There was no significant difference in the percentage before and after training. However, before and after training, the percentage of theta rhythms and the amplitude (intensity: + 2.5-2.5 db) in the right CA3 area were significantly decreased (P <0.01) . The results of this study showed that the percentage of β2 and γ1 rhythms in the right hippocampal CA3 region and the rhythms of θ rhythm remained relatively high after fast-avoidance trauma-shock training in rats, and these changes may be related to their strong spatial cognition.