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AIM: To observe the variation of DNA polymerase (3 (polp) in esophageal carcinoma. METHODS: Thirty specimens containing adjacent normal epithelial tissues were collected from patients in Linzhou region (a high risk area for esophageal squamous carcinoma) and 25 specimens were from a non-high risk area. Total RNA was extracted from the samples and reverse transcription polymerase chain reaction (RT-PCR) was performed. PCR products were cloned and sequenced to investigate the polp gene with DNASIS and OMIGA. Statistical significance was evaluated using the X2 test. RESULTS: High-incidence area group: polp gene variation was detected in 13 of 30 esophageal carcinoma tissue specimens, and only one variation was found in 30 corresponding adjacent normal tissue specimens. Non high-incidence area group: polp gene variation was detected in 5 of 25 esophageal carcinoma tissue specimens, and no variation was found in 25 corresponding adjacent normal tissue specimens. The incidence of polp gene variation observed in the high-incidence area group was significantly higher than in the non-high incidence area group. Two mutation hot spots (454-466 and 648-670 nt) and a 58 bp deletion (177-234 nt) were found. CONCLUSION: Variations of polp perform different functions between the high-incidence areas and the other areas, and may play a more important role in the high-incidence areas.
AIM: To observe the variation of DNA polymerase (3 (polp) in esophageal carcinoma. METHODS: Thirty specimens containing adjacent normal epithelial tissues were from patients in Linzhou region (a high risk area for esophageal squamous carcinoma) and 25 specimens were from from a Total RNA was extracted from the samples and reverse transcription polymerase chain reaction (RT-PCR) was performed. PCR products were cloned and sequenced to investigate the polp gene with DNASIS and OMIGA. Statistical significance was evaluated using the X2 test. RESULTS: High-incidence area group: polp gene variation was detected in 13 of 30 esophageal carcinoma tissue specimens, and only one variation was found in 30 corresponding adjacent normal tissue specimens. in 5 of 25 esophageal carcinoma tissue specimens, and no variation was found in 25 corresponding adjacent normal tissue specimens. The incidence of polp gene v Both mutation hot spots (454-466 and 648-670 nt) and a 58 bp deletion (177-234 nt) were found. CONCLUSION: Variations of polp perform different functions between the high-incidence areas and the other areas, and may play a more important role in the high-incidence areas.