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Objective: To study the effect of electroacupuncture(EA)of Zusanli (ST 36) on plasma epidermal growth factor (EGF) and calcitonin gene related peptide (CGRP) levels in stress rats. Methods: 40 Wistar rats were randomly divided into control group (n=8) which was not given with either stress or EA; stress group (n=8) which was given only with stress stimulation, and EA plus stress group (divided into 3 subgroups 1 d, 3 d and 5 d according to the EA treatment duration 1 day, 3 days and 5 days, with 8 rats being in each subgroup). The restrained cold stress method was used for making stress animal model. Under light microscope and transmission electron microscope, the structural changes of the gastric mucosa were observed. By radioimmunoassay and Guth methods,plasma EGF and CGRP contents and the related ulcer index (UI) were analyzed. Results: In comparison with control group, plasma EGF of stress group decreased significantly (0.44±0.16 μg/L vs 0.23±0.01 μg/L, P<0.05); while UI increased considerably (1.10±0.40 vs 28.0±4.1, P<0.01). Compared with stress group, both plasma EGF and plasma CGRP in EA subgroup 1d increased significantly (0.23±0.01 μg/L vs 0.42±0.09 μg/L, P<0.05;145.00±6.00 ng/L vs 184.00±22.00 ng/L, P<0.05 respectively); while UI lowered obviously (28.00±4.10 vs 19.00±2.30, P<0.01). Plasma CGRP content of subgroup 5d increased significantly in comparison with that of subgroup 1d (184.00±22.00 ng/L vs 232.00±35.00 ng/L, P<0.01). Compared with EA group, the degrees of necrosis and damages of the mucosal cells and vascular endothelium were more severe in stress group. Conclusion: EA of Zusanli (ST 36) can protect gastric mucosa from injury in stress rats, in which the periphery EGF and CGRP are involved and the regulatory action of EA on plasma CGRP is also related to the treatment duration.