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为了解子宫内膜异位症(EMs)与腹腔和外周血中B-1细胞数量的关系,检查了31例EMs患者(年龄28.3±7.3岁)和14例正常未孕女性(年龄27.7±8.5岁)的外周血标本和腹腔细胞,并对其用荧光素异硫氰酸盐(FITC)或PE标记的抗CD5/CD 19的单克隆抗体染色,用激光流式细胞计数仪行免疫荧光分析,用HEp-2细胞作为底物,通过间接荧光法检测抗核抗体。用非参数Mann-Whitney检验行统计学处理。 结果:①有抗核抗体的EMs患者,外周血中44.6％的CD19染色的B细胞同时表达CD5,而正常个体中仅有21.1％。②EMs患者(n=13)腹腔渗出物细胞(PECs)中的
To investigate the relationship between endometriosis (EMs) and the number of B-1 cells in peritoneal and peripheral blood, 31 patients with EMs (age 28.3 ± 7.3 years) and 14 normal nonpregnant women (age 27.7 ± 8.5 Year) peripheral blood cells and peritoneal cells, and stained with fluorescein isothiocyanate (FITC) or PE-labeled anti-CD5 / CD 19 monoclonal antibody, and subjected to immunofluorescence analysis with a laser flow cytometer , Anti-nuclear antibodies were detected by indirect fluorescence using HEp-2 cells as a substrate. Statistical analysis was performed using a nonparametric Mann-Whitney test. Results: ① In anti-nuclear antibody-resistant patients, 44.6% of CD19-stained B cells expressed CD5 in peripheral blood, compared with 21.1% in normal individuals. ②EMs patients (n = 13) in peritoneal exudate cells (PECs)