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目的 :探讨富硒米喂养的大鼠全血硒水平与免疫功能的关系 ,为富硒米用于防癌保健提供科学根据。方法 :30天喂养试验 :2、3-二氨基萘荧光分析法测全血硒水平 ;3 H- Td R掺入液闪法检测淋巴细胞转化。结果 :低、中、高剂量组及对照组的大鼠全血硒水平分别为 0 .575± 0 .1 54,0 .686± 0 .2 0 6,0 .894± 0 .1 80 ,0 .479± 0 .0 88μg/ml,且中、高剂量组显著性地高于对照组( P<0 .0 5,P<0 .0 1 ) ;低、中、高剂量组的淋巴细胞转化分别为 0 .762± 0 .461 ,0 .887± 0 .474,0 .791± 0 .539,0 .42 4± 0 .2 2 1 cpm万 /1 0 6细胞 ,且中剂量组显著性地高于对照组 ( P<0 .0 5) ;受试动物进食、活动等正常 ,动物体重、血常规亦正常 ,未见动物中毒和死亡情况。结论 :富硒米可提高受试动物的全血硒水平 ,血硒水平的提高有利于增强机体的免疫功能。富硒米不引起动物中毒和死亡 ,提示富硒米为无毒食品 ,可用于防癌保健。
Objective : To investigate the relationship between selenium levels and immune function in selenium-enriched rice-fed rats and to provide scientific basis for anti-cancer health care for selenium-rich rice. Methods: 30-day feeding experiment: 2,3-diaminonaphthalene fluorescence assay was used to measure the level of selenium in the whole blood; 3 H-Td R was added to the liquid scintillation assay to detect lymphocyte transformation. RESULTS: The selenium levels in whole blood of rats in the low-, medium-, and high-dose group and the control group were 0.575± 0 .1 54 0. 686.±.0.26. 0.894± 0 .1 80 , 0 respectively. It was significantly higher than that of the control group (P<0.05, P<0.01). The lymphocyte transformation was observed in the low-medium, high-dose groups. They were 0.762± 0 .461, 0.887± 0.474, 0.791±0.549, 0.424±0.22 cpm/106 cells, respectively, and they were significant in the middle-dose group. Compared with the control group (P<0.05), the animals were normal in eating and activities, and the body weight and blood routine of the animals were normal. There was no animal poisoning and death. Conclusion : Selenium-enriched rice can increase the selenium level in whole blood of test animals. The increase of blood selenium level is beneficial to enhance the body’s immune function. Selenium-enriched rice does not cause animal poisoning and death, suggesting that selenium-enriched rice is a non-toxic food and can be used for anti-cancer health care.