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研究了水蒲桃核仁多糖(PoIysaccharide of Syzygium jambos(Linn.)Alston Seeds,PSJA)水提过程中提取温度、液料比、提取时间、提取次数等因素对其提取率的影响,运用响应面设计试验确定水提PSJA的最佳提取工艺,得出水提法提取PSJA的适宜条件为:提取温度为75℃,液料比16:1(mL/g),提取时间2.9 h,提取次数2次,在此条件下测得PSJA的提取率为6.25%。进一步利用乙醇沉淀获得多糖,当乙醇体积分数为75%时水蒲桃核仁多糖的沉淀率最大,在此条件下多糖的得率为5.12%,纯度为70.7%。
The effects of extraction temperature, liquid to solid ratio, extraction time, extraction times and other factors on the extraction rate of PoIysaccharide of Syzygium jambos (Linn.) Alston Seeds (PSJA) were studied. The response surface The optimal extraction conditions of water-extracted PSJA were determined by design test. The optimum conditions for extracting PSJA by water extraction were as follows: extraction temperature 75 ℃, liquid-to-liquid ratio 16: 1 (mL / g), extraction time 2.9 h, extraction times 2 Under this condition, the extraction rate of PSJA was 6.25%. The polysaccharides were further precipitated by ethanol precipitation. When the volume fraction of ethanol was 75%, the precipitation rate of water polysaccharides was the highest. Under this condition, the yield of polysaccharides was 5.12% and the purity was 70.7%.