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目的研究精浆中胱抑素C(CysC)的生物学特性及其检测意义,建立可供临床研究的参考数据。方法应用免疫胶体金技术对192例不育患者精浆及50例正常生育的男性精浆CysC进行对比检测,同时监测50例正常对照精浆标本在不同温度及时间段内CysC的变化情况。结果192例患者精浆标本中CysC最低值为6.53mg/L,最高值为43.56mg/L,平均值为(19.37±7.43)mg/L;50例正常对照精浆中最低CysC值为18.13mg/L,最高CysC值为28.84mg/L,平均值为(23.55±2.50)mg/L。少精子症为(15.92±4.96)mg/L、弱精子症为(15.62±2.13)mg/L、死精子症为(15.26±2.12)mg/L、无精子症为(13.88±2.38)mg/L、血精症为(15.52±5.92)mg/L、脓精症为(19.45±9.87)mg/L、精液不液化为(13.99±3.88)mg/L;患者各组间与正常对照组CysC值经统计学分析差异具有统计学意义(P<0.05)。精液中WBC数<5个/HP的CysC值最低为6.53mg/L,最高为34.82mg/L,平均值为(16.40±5.75)mg/L;精液中WBC数为5～10个/HP的CysC值最低为8.95mg/L,最高为39.54mg/L,平均值为(19.75±6.05)mg/L;精液中WBC数>10个/HP的CysC值最低为10.73mg/L,最高为43.56mg/L,平均值为(21.96±10.49)mg/L。结论少精子症、弱精子症、无精子症、死精子症、血精症、脓精症、精液不液化患者精浆CysC总体水平均明显低于正常对照组。炎症精浆中CysC值的变化提示CysC可能与其主要的细胞学来源及炎症部位相关。
Objective To study the biological characteristics of cystatin C (CysC) in seminal plasma and the significance of its detection, and establish reference data for clinical research. Methods Immunocytose gold technique was used to detect CysC in seminal plasma of 192 infertile patients and in 50 normal seminal plasma of male patients. Meanwhile, the changes of CysC in 50 normal seminal plasma samples at different temperature and time were monitored. Results The lowest value of CysC in the seminal plasma of 192 patients was 6.53mg / L, the highest value was 43.56mg / L, the average value was (19.37 ± 7.43) mg / L. The lowest CysC value of seminal plasma was 18.13mg / L, the highest CysC value was 28.84mg / L, the average value was (23.55 ± 2.50) mg / L. (15.92 ± 4.96) mg / L for oligospermia, (15.62 ± 2.13) mg / L for asthenospermia, (15.26 ± 2.12) mg / L for spermatozoa and (13.88 ± 2.38) mg / (15.52 ± 5.92) mg / L, (19.45 ± 9.87) mg / L for seminal fluid, and (13.99 ± 3.88) mg / L for semen. The CysC The difference was statistically significant (P <0.05). The lowest CysC value of semen WBC <5 / HP was 6.53mg / L, the highest was 34.82mg / L, the average was (16.40 ± 5.75) mg / L; the number of semen WBC was 5 ~ 10 / HP The lowest CysC value was 8.95mg / L, the highest was 39.54mg / L, the average was (19.75 ± 6.05) mg / L; the lowest CysC value was 10.73mg / L and the highest was 43.56 mg / L, with an average of (21.96 ± 10.49) mg / L. Conclusions The level of CysC in seminal plasma of oligozoospermia, asthenospermia, azoospermia, spermatozoa, apomorphosis, sepsis and seminal fluid in patients with liquefaction is significantly lower than that of normal control group. Changes in CysC values in inflammatory seminal fluid suggest that CysC may be related to its major cytological origins and inflammatory sites.