论文部分内容阅读
目的:研究银杏叶提取物的胃粘膜保护作用.方法:采用大鼠束缚-冷冻应激(RCS)模型和小鼠无水乙醇损伤模型观察GbE对胃粘膜损伤指数的影响;采用幽门结扎法收集胃液,观察GbE对胃液分泌量,胃液酸度和胃蛋白酶活性的影响;采用硫代巴比妥酸(TBA)法测定胃粘膜及血清中丙二醛(MDA)含量.结果:GbE(25,50,100 mg/kg,bid×5 d,ig)剂量依赖性地抑制RCS和无水乙醇引起的胃粘膜损伤.用药组应激后的胃粘膜损伤指数分别为对照组的58％,43％和31％;用药组乙醇诱发的胃粘膜损伤指数降至对照组的62％,36％和26％;GbE尚能增强西米替丁对胃粘膜的保护作用,但对大鼠胃液分泌量、胃液酸度及胃蛋白酶活性GbE并无明显影响.小鼠经无水乙醇ig后1 h,胃粘膜和血清中的MDA含量显著升高(P<0.01),而GbE(25,50,100 mg/kg,ig)预处理则可以明显抑制MDA的升高.结论:GbE具有胃粘膜保护作用,并且与西米替丁在治疗急性胃粘膜损伤方面具有协同作用.
Objective: To study the gastric mucosal protective effect of Ginkgo biloba extract. Methods: Observe the effect of GbE on the gastric mucosal injury index by using rat restraint-freezing stress (RCS) model and mouse absolute alcohol injury model; collect with pylorus ligation Gastric juice was used to observe the effect of GbE on gastric juice secretion, gastric juice acidity and pepsin activity; thiobarbituric acid (TBA) method was used to measure malondialdehyde (MDA) content in gastric mucosa and serum. Result: GbE(25,50,100) Mg/kg, bid x 5 d, ig) Gastric mucosal damage induced by RCS and ethanol in a dose-dependent manner. The gastric mucosal injury index after treatment in the treatment group was 58%, 43% and 31%, respectively, in the control group. The alcohol-induced gastric mucosal injury index of the drug group decreased to 62%, 36%, and 26% of the control group; GbE still enhanced the protective effect of cimetidine on the gastric mucosa, but the secretion of gastric juice and the acidity of gastric juice in rats were increased. Pepsin activity GbE did not have a significant effect. One hour after ethanol ig mice, MDA content in gastric mucosa and serum increased significantly (P <0.01), while GbE (25,50,100 mg/kg, ig) pre Treatment can significantly inhibit the elevation of MDA. Conclusion: GbE has gastric mucosal protection and is treated with cimetidine. Gastric mucosal injury in terms of a synergistic effect.