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目的:探讨PCA3基因多态性与前列腺癌遗传易感性的关系。方法:采用PCR和基因片段直接测序方法,分别检测41例前列腺癌(PCa)患者和40例良性前列腺增生(BPH)患者的PCA3外显子区域的SNP变异情况,分析PCA3基因多态性与PCa的相关性。结果:PCA3基因外显子2区域存在1个SNP位点(A164C),基因型分别为AA型、AC型、CC型;Logistic回归分析显示,AC、CC及AC+CC基因型是PCa的危险因子(OR值=6.171,95%CI=1.877-20.286;OR值=5.400,95%CI 1.421-20.518;OR值=5.891,95%CI=1.915-18.124),等位基因C是PCa的危险因子(OR值=2.353,95%CI=1.252-4.421)。结论:PCA3基因外显子2 SNP A164C多态性与PCa的发病风险高度相关,携带等位基因C的基因型是PCa的遗传危险因素。
Objective: To investigate the relationship between PCA3 gene polymorphism and genetic susceptibility to prostate cancer. Methods: SNPs in exon of PCA3 in 41 patients with prostate cancer (PCa) and 40 patients with benign prostatic hyperplasia (BPH) were detected by PCR and gene fragment direct sequencing. The association between PCA3 polymorphism and PCa Relevance. Results: There was one SNP locus (A164C) in exon 2 of PCA3 gene. The genotypes were AA, AC and CC. Logistic regression analysis showed that the AC, CC and AC + CC genotypes were the risk of PCa (OR = 6.171, 95% CI = 1.877-20.286; OR = 5.400, 95% CI 1.421-20.518; OR = 5.891,95% CI = 1.915-18.124), allele C was a risk factor for PCa (OR value = 2.353, 95% CI = 1.252-4.421). Conclusion: The exon 2 SNP A164C polymorphism of PCA3 gene is highly correlated with the risk of PCa. The genotype of allele C is a genetic risk factor of PCa.