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用PCR(Polymerase Chain Reaction)扩增出天花粉蛋白(Trichosanthin,TCS)基因全长序列,插入表达载体.经双酶切鉴定及测序分析,表明阅读框正确.表达His-TCS活性融合蛋白并纯化天花粉蛋白,鉴定出其具有RNA N-糖苷酶活性和抗原抗体结合活性.
天花粉蛋白基因的克隆、表达和活性鉴定
【摘 要】
：
用PCR(Polymerase Chain Reaction)扩增出天花粉蛋白(Trichosanthin,TCS)基因全长序列,插入表达载体.经双酶切鉴定及测序分析,表明阅读框正确.表达His-TCS活性融合蛋白并纯化
【机 构】
：
上海交通大学,中国科学院
【出 处】
：
上海交通大学学报
【发表日期】
：
2004年期
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