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目的:研究佩兰中药颗粒对2型糖尿病大鼠糖脂代谢的改善作用,探讨中药佩兰对大鼠肝脏DGAT2表达的影响。方法:高脂饮食加尾静脉小剂量注射链脲佐菌素(STZ 28 mg/kg)建立2型糖尿病大鼠模型,将造模成功的合并脂代谢紊乱的2型糖尿病大鼠随机分为阳性对照组、佩兰中药颗粒组、吡格列酮组每组9只,另设空白对照组10只。佩兰中药颗粒组予佩兰中药颗粒按3 g/(kg·d)灌胃给药,吡格列酮组0.3 mg/(kg·d)灌胃,阳性对照组及空白对照组予0.9%氯化钠注射液灌胃,持续给药5周后,检测血清甘油三酯(serum triglyceride,TG)、血清总胆固醇(serum total cholesterol,TC)、游离脂肪酸(free fatty acid,FFA)、空腹血糖(fasting blood glucose,FBG)、空腹胰岛素(fasting insulin,FINS)并计算HOMA-IR评估大鼠胰岛素抵抗水平。应用RT-PCR与Western blot方法检测肝脏DGAT2基因及蛋白表达水平。结果:经治疗后,佩兰中药颗粒组大鼠的血清TG、TC水平与阳性对照组相比得到了明显的改善,肝脏DGAT2的基因及蛋白表达水平与阳性对照组相比下调。结论:佩兰可以改善由链脲佐菌素诱导的2型糖病大鼠脂代谢紊乱,其机制与中药佩兰下调大鼠肝脏中DGAT2基因与蛋白表达有关。
OBJECTIVE: To study the effect of Perrin Chinese traditional medicine granule on glucose and lipid metabolism in type 2 diabetic rats and to explore the effect of Perilla on rat liver DGAT2 expression. Methods: A rat model of type 2 diabetes mellitus was established by injecting streptozotocin (STZ 28 mg / kg) into the tail vein of a high-fat diet. The rats with type 2 diabetes mellitus with successful lipid metabolism disorders were randomly divided into positive The control group, Perrin Chinese medicine granule group, pioglitazone group, each group of 9, and the other 10 blank control group. Perrin Chinese Granules Granule Peilan by gavage at 3 g / (kg · d), pioglitazone group 0.3 mg / (kg · d) gavage, positive control group and blank control group 0.9% sodium chloride injection After 5 weeks of continuous administration, serum triglyceride (TG), serum total cholesterol (TC), free fatty acid (FFA), fasting blood glucose FBG, fasting insulin (FINS) were calculated and HOMA-IR was used to evaluate the level of insulin resistance in rats. The liver DGAT2 gene and protein expression levels were detected by RT-PCR and Western blot. Results: After treatment, the levels of serum TG and TC in Perrin Chinese Granules group were significantly improved compared with the positive control group. The gene and protein expression level of DGAT2 in liver decreased compared with the positive control group. Conclusion: Perrin can improve the disorder of lipid metabolism induced by streptozotocin in type 2 diabetic rats, and its mechanism is related to the down-regulation of DGAT2 gene and protein expression in rat liver by Perrin.