论文部分内容阅读
红麻是一种以收获韧皮纤维为主的经济作物,因其具有纤维产量高,抗逆境生长能力强等特点,在我国大部分地区都有种植。近年来,随着其韧皮纤维在纺织工业中的应用,红麻越来越受到育种家的高度重视。其韧皮纤维中木质素含量高低是限制其纤维品质好坏的重要因素之一,过氧化物酶基因在不同品质的木材中差异表达较为显著。为研究该基因在红麻(Hibiscus cannabinus L.)木质素合成过程中的作用,通过红麻转录组数据库筛选到过氧化物酶(Peroxidase)基因的核心片段,利用Tail-PCR技术获得了Peroxidase基因c DNA全长,命名为Hc POD。生物信息学分析表明:Hc POD基因编码序列长952 bp,编码316个氨基酸,相对分子量为33.9 k D,等电点为4.85。为进一步研究该基因的功能,本研究利用p CAMBIA1301载体作为骨架载体构建了红麻过氧化物酶基因过表达载体,利用花絮侵染法转化拟南芥。通过对转基因和非转基因植株体内木质素含量检测后发现,在拟南芥体内过表达红麻POD基因能显著提高拟南芥的木质素含量水平。研究结果为进一步分析该基因功能和了解红麻木质素合成的分子机制具有意义。
Kenaf is a kind of economic crops mainly balsam fiber harvest, because of its high fiber yield, anti-stress ability to grow strong, in most parts of China have planted. In recent years, with the application of bast fibers in the textile industry, kenaf is more and more valued by breeders. The content of lignin in the bast fiber is one of the important factors that limit the quality of its fiber. The peroxidase gene is significantly different in different quality of wood. In order to study the role of this gene in the lignin synthesis of Kenaf (Hibiscus cannabinus L.), the core fragment of Peroxidase gene was screened from the kenaf transcriptome database and the Peroxidase gene was obtained by Tail-PCR c DNA full length, named Hc POD. Bioinformatics analysis showed that the coding sequence of Hc POD gene was 952 bp in length, encoding 316 amino acids with a relative molecular mass of 33.9 kD and an isoelectric point of 4.85. In order to further study the function of this gene, we constructed the over-expression vector of kenaf peroxidase gene using p CAMBIA1301 vector as backbone vector, and transformed Arabidopsis thaliana with interspersed infection method. Through the detection of lignin content in transgenic and non-transgenic plants, it was found that overexpression of kenaf POD gene in Arabidopsis can significantly increase the lignin content of Arabidopsis thaliana. The results of this study are of great significance for further analysis of the gene function and understanding of the molecular mechanism of kenaf lignin synthesis.