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目的 比较腹腔灌洗液中端粒酶活性和细胞学检测游离癌细胞及预测腹膜转移的应用价值。方法 应用端粒重复扩增 (TRAP) -酶联免疫吸附测定 (ELASA)法检测 5 2例胃癌和 12例胃溃疡患者腹腔灌洗液中的端粒酶活性 ,同时对 5 2例胃癌患者行腹腔灌洗液脱落细胞学 (peritoneallavagecytology ,PLC)检测。 结果 12例胃溃疡患者腹腔灌洗液中端粒酶活性均为阴性。胃癌患者腹腔灌洗液中端粒酶活性的阳性率随侵袭深度、病期进展而增加 (P <0 0 5 )。TRAP ELISA方法的阳性率比脱落细胞学检测显著增高(48 1%比 2 5 0 % ,P <0 0 5 )。结论 两种方法均适用于胃癌腹腔脱落癌细胞的诊断或腹膜转移的预测。但对微量癌细胞检出的灵敏度 ,以端粒酶活性检测为优
Objective To compare the telomerase activity in peritoneal lavage fluid and the cytological detection of free cancer cells and to predict the value of peritoneal metastasis. Methods Telomerase activity in peritoneal lavage fluid of 52 patients with gastric cancer and 12 patients with gastric ulcer was detected by telomeric repeat amplification (TRAP) -ELISA method. Meanwhile, 52 patients with gastric cancer Peritoneal lavage cytology (PLC) test. Results The telomerase activity in peritoneal lavage fluid of 12 patients with gastric ulcer was negative. The positive rate of telomerase activity in peritoneal lavage fluid of patients with gastric cancer increased with the invasion depth and disease progression (P <0.05). The positive rate of TRAP ELISA was significantly higher than that of exfoliative cytology (48 1% vs 250%, P 0 05). Conclusion Both methods are suitable for the diagnosis of peritoneal cancer cells or the prediction of peritoneal metastasis of gastric cancer. However, the sensitivity of detection of trace cancer cells, detection of telomerase activity is excellent