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目的了解佛山市副溶血弧菌病原学与分子流行病学特征,为副溶血弧菌感染暴发的早期预警、分子流行病学调查及溯源提供准确的实验室数据。方法选取监测食品、食物中毒、散发病例来源的副溶血弧菌,采用血清学、药物敏感性试验、多重PCR检测毒力基因和PFGE分子分型等方法,分析不同来源的副溶血弧菌病原学特征以及分子分型方面的异同。结果 151株菌株中,监测食品分离株血清型分布呈现多样性,无优势菌株;食物中毒分离株和散发病例分离株的优势血清型为O3∶K6。监测食品、食物中毒和散发病例分离株对氨苄西林和磺胺嘧啶都有较高的耐药率,多重耐药情况比较严重。监测食品分离株99.18%(121/122)为tdh-trh-菌株,1株tdh+trh+菌株;食物中毒分离株中tdh+trh-菌株占69.23%(9/13),30.77%(4/13)tdh-trh-菌株;散发病例分离株均为tdh+trh-菌株。151株菌株共获得134个不同的PFGE带型,监测食品分离株的PFGE呈现遗传多样性,无优势带型,并且与散发及食物中毒患者分离株的PFGE带型不同。食物中毒与散发病例分离株带型相对比较集中。结论佛山市不同来源的副溶血弧菌血清型、毒力基因和遗传特征复杂多样,菌株多重耐药形势严峻,需要加强菌株型别变异及耐药监测。
Objective To understand the etiology and molecular epidemiology of Vibrio parahaemolyticus in Foshan and to provide accurate laboratory data for early warning, molecular epidemiological investigation and traceability of Vibrio parahaemolyticus infection. Methods Vibrio parahaemolyticus (Vibrio parahaemolyticus) was selected for surveillance of food and food poisoning. Serological and drug susceptibility tests were performed to detect virulence genes and PFGE molecular typing by multiplex PCR. The pathogenicity of Vibrio parahaemolyticus Similarities and differences in characteristics and molecular typing. Results Among the 151 strains, the distribution of serotypes of foodstuff isolates showed diversity and no predominant strains. The dominant serotypes of food poisoning isolates and isolates were O3: K6. Monitoring food, food poisoning and sporadic cases of isolates of ampicillin and sulfadiazine have a higher resistance rate, multi-drug resistance is more serious. Among the food poisoning isolates, tdh-trh-strain and tdh + trh + strain accounted for 69.18% (9/13) and 30.77% (4/13), respectively, in 99.18% (121/122) ) tdh-trh-strain; sporadic case isolates are tdh + trh- strains. A total of 134 strains of PFGE were obtained from 151 strains. The genetic diversity of PFGE in the monitoring food isolates showed no genetic predominance, which was different from PFGE patterns of isolates of sporadic and food poisoning strains. Food poisoning and sporadic cases isolated strains relatively concentrated. Conclusion The serotypes, virulence genes and genetic characteristics of Vibrio parahaemolyticus from different sources in Foshan are complex and diverse. The situation of multiple drug resistance in strains is severe, so strain type variation and drug resistance monitoring should be strengthened.