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目的观察Ki67、P53、nm23在不同病理学特征的大肠癌中表达情况,分析其与肿瘤分化、浸润及转移方面的关系。方法选取2004年1月~2012年9月在我院进行手术治疗的74例大肠癌患者切除标本,采用免疫组化法观察其中Ki67、P53、nm23的表达。结果本次研究中nm23基因总阳性率为62.16%,其表达与肿瘤分化程度无关(P>0.05),但与肿瘤浸润深度(P<0.05)和淋巴转移(P<0.01)密切相关;P53基因总阳性率为52.7%,其表达与肿瘤分化程度(P<0.01)、浸润深度(P<0.01)和淋巴转移(P<0.05)均存在密切联系;Ki67蛋白总阳性率为74.32%,其表达与肿瘤分化程度(P>0.05)没有肯定联系,但其与肿瘤浸润深度及是否出现淋巴转移密切相关(P<0.05)。结论 Ki67、P53、nm23的表达对于大肠癌的肿瘤分化、浸润及转移具有不同程度影响,对上述指标的观察有助于指导大肠癌的治疗和预后评估。
Objective To observe the expression of Ki67, P53 and nm23 in colorectal cancer with different pathological features, and to analyze their relationship with tumor differentiation, invasion and metastasis. Methods Totally 74 patients with colorectal cancer who underwent surgery in our hospital from January 2004 to September 2012 were selected for resection. The expressions of Ki67, P53 and nm23 were observed by immunohistochemistry. Results The positive rate of nm23 gene in this study was 62.16%. The expression of nm23 gene was not correlated with the degree of tumor differentiation (P> 0.05), but closely correlated with the depth of tumor invasion (P <0.05) and lymphatic metastasis (P <0.01) The positive rate of Ki67 protein was 52.7%. The expression of Ki67 protein was closely related to the degree of differentiation (P <0.01), depth of invasion (P <0.01) and lymph node metastasis (P <0.05) There was no relationship between tumor differentiation and tumor differentiation (P> 0.05), but it was closely related to the depth of tumor invasion and lymphatic metastasis (P <0.05). Conclusion The expressions of Ki67, P53 and nm23 have different effects on the differentiation, invasion and metastasis of colorectal cancer. The observation of these indexes can help to guide the treatment and prognosis evaluation of colorectal cancer.