论文部分内容阅读
目的 观察不同速率生长的胶质瘤细胞的葡萄糖代谢状态。方法 用C6细胞克隆培养得到不同细胞形态、不同生长速率的细胞株。分别检测生长最快的C64细胞和最慢的C61细胞的胞内葡萄糖、ATP浓度 ,同时检测两种细胞的己糖激酶蛋白和它们的胞质 /线粒体己糖激酶活性。结果 C64细胞内检测不到葡萄糖 ,C61细胞内葡萄糖浓度为 (0 .17± 0 .0 5 ) μmol/g蛋白 ;C64细胞、C61细胞内ATP浓度分别为 (10 .16± 1.5 1) μmol/g蛋白和 (7.5 7± 0 .3 2 ) μmol/g蛋白。条带的吸光度分析显示C64细胞的己糖激酶Ⅰ稍高于C61细胞 ;C64细胞线粒体己糖激酶活性约为C61细胞的 3倍 ,但其胞质己糖激酶活性仅为C61细胞的一半。结论 葡萄糖转运系统与肿瘤细胞的葡萄糖摄取、葡萄糖代谢状态及生长速度等密切相关。
Objective To observe the glucose metabolism status of glioma cells growing at different rates. Methods Cell lines with different cell morphology and growth rates were obtained by cloning C6 cells. The intracellular glucose and ATP concentrations of the most rapidly growing C64 cells and the slowest C61 cells were respectively detected, and the hexokinase proteins and their cytoplasmic / mitochondrial hexokinase activities of both cells were simultaneously detected. Results Glucose was not detected in C64 cells. The intracellular glucose concentration in C61 cells was (0.17 ± 0.05) μmol / g protein. The intracellular ATP concentrations in C64 cells and C61 cells were (10.16 ± 1.51) μmol / g protein and (7.57 ± 0.32) μmol / g protein. Absorbance analysis showed that the hexokinase Ⅰ of C64 cells was slightly higher than that of C61 cells. The mitochondrial hexokinase activity of C64 cells was about 3 times higher than that of C61 cells, but its cytoplasmic hexokinase activity was only half that of C61 cells. Conclusion Glucose transport system is closely related to the glucose uptake, glucose metabolism status and growth rate of tumor cells.