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彩叶凤尾蕨(Pteris aspericaulis)又名三色凤尾蕨,生长在云南南部,它是一种稀有的观赏植物。叶片纵向呈红、白、绿三色条纹,在湿度大、温度25℃以上的条件下,三种色彩更为鲜明(见封四)试验材料为长有孢子束的野生叶片。将叶片消毒,在无菌条件下,将孢子束群盖剥开,取孢子束接人加有细胞分裂素和生长素的MS培养基中培养,温度25一28℃,光照1。小时,光强500lx。约经3个月时间,抱子萌发并长出假根,逐步长成丝状体。
Pteris aspericaulis (Pteris aspericaulis), also known as trichosanthes Pteridophyte, grow in southern Yunnan, it is a rare ornamentals. The leaves are red, white and green stripes in the longitudinal direction. Under the conditions of high humidity and temperature above 25 ℃, the three colors are more vivid (see seal IV). The test materials are wild leaves with long spores. The leaves were sterilized, and under sterile conditions, the spore bunch cover was peeled off, and the spore bundle was picked up and cultured in MS medium supplemented with cytokinin and auxin at a temperature of 25 to 28 ° C and irradiated with light 1. Hours, light 500lx. After about 3 months, sprout germination and grow a false root, and gradually grow into filaments.