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为了解绵羊和山羊嗜血支原体在甘肃、辽宁、内蒙古、宁夏、重庆、海南等地的流行状况及病原种类,利用瑞氏染色方法和已发表的嗜血支原体通用qPCR方法,对2011—2015年间采自上述地区的绵羊和山羊血液样品346份进行了检测,并对部分qPCR阳性样品扩增产物进行了测序和序列分析。结果,在甘肃、内蒙古的绵羊样品中和重庆、海南的山羊样品中检出嗜血支原体;其中,瑞氏染色方法的绵羊样品阳性率为12.45%(33/265),山羊样品阳性率为24.69%(20/81);qPCR绵羊阳性率为22.26%(59/265),山羊阳性率为37.04%(30/81)。对部分阳性样品的qPCR产物进行的序列分析结果显示,绵羊样品中5/7份为绵羊支原体和绵羊嗜血支原体待定种共同感染,1/7为绵羊支原体感染,1/7为绵羊嗜血支原体待定种感染;山羊样品中,2/3为绵羊支原体感染,1/3为绵羊嗜血支原体待定种感染。本文在我国首次证实山羊和绵羊中流行绵羊支原体和绵羊嗜血支原体待定种两种不同的嗜血支原体,且绵羊中存在两种嗜血支原体共同感染的情况。
In order to understand the prevalence and pathogens of sheep and goat M. hyopneumoniae in Gansu, Liaoning, Inner Mongolia, Ningxia, Chongqing, Hainan and other places, Wright’s stain method and the published M. thromboplastin universal qPCR method were used to analyze the prevalence of Mycoplasma hyopneumoniae between 2011-2015 346 samples of sheep and goat blood samples collected from the above areas were tested, and some qPCR positive sample amplification products were sequenced and sequence analysis. As a result, M.pneumoniae was detected in sheep samples in Gansu and Inner Mongolia and goat samples in Chongqing and Hainan. The positive rate of Sheep’s stain was 12.45% (33/265) and the positive rate of goat was 24.69 % (20/81). The positive rate of qPCR sheep was 22.26% (59/265) and the positive rate of goats was 37.04% (30/81). The sequence analysis of qPCR products of some positive samples showed that 5/7 samples of sheep were co-infected with Mycoplasma ovipneumoniae and M. hyopneumoniae, 1/7 were Mycoplasma oviposition and 1/7 were M. hyopneumoniae To be scheduled for infection; goat samples, 2/3 sheep Mycoplasma infection, 1/3 of Mycoplasma hyopneumoniae to be scheduled for infection. This article for the first time confirmed mycoplasma and Mycoplasma hyopneumoniae in goat and sheep to be determined two kinds of Mycoplasma hyopneumoniae in sheep and co-infection of two kinds of Mycoplasma hyopneumoniae in sheep.