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利用具有提莫菲维细胞质的雄性不育“克里斯”(普通小麦)的单体组对六个普通小麦恢复系中的育性恢复基因进行染色体定位。此六个恢复系为:R1—李(内布拉斯加542437号与普通小麦“李”的衍生种),R2—索诺拉64(提莫菲维小麦与普通小麦“马奎斯”~3、“索诺拉64”的衍生种)、R3(提莫菲维小麦与普通小麦“马奎斯”~3的衍生种)、R4(提莫菲维小麦和山羊草属Ae.Squarrosa的双二倍体和普通小麦“德克”~3的衍生种)、R5(茹可夫斯基和普通小麦“贾斯廷”~3的衍生种)和普通小麦“普里麦皮”。自单体的F_1衍生的F_2和F_3中雄性不育和可育植株的相互比例表明:R1—李、R2—索诺拉64、R3、R4和R5的1A和7D染色体上均具有育性恢复基因(以下均以Rf表示—译注)。R2—索诺拉64和R5还各有一个Rf,分别位于染色体6B和7B上。“普里麦皮’’在1B和5D染色体上有Rf。对照和一些非甄别衍生种育性恢复的遗传表明:R1—李、R2—索诺拉64和R5各具有三个Rf,而R3、R4和“普里麦皮”则各有二个。某些染色体的缺失影响了一些衍生种Rf的外显率和表现度。有几位工作者用单体分析法来确定普通小麦中Rf在染色体上的位置。从“马奎斯”~3与提莫菲维小麦杂交获得的一个恢复系(R3)在1A染色体上有一个Rf。第二个Rf位于R3的7D染色体上。同样,Talaat(1969)报导过R1—李和R4在1A和7D上具有Rf,且在5A上具有一个弱效应的Rf。R3和R4的1B染色体具有一个育性抑制基因。然而,斯卑尔脱小麦“杜哈迈里阿南”和普通小麦“明尼斯特”在1B染色体上有一个Rf。Yen,Evans和Latter对由提莫菲维小麦和山羊草属Ae、Squarrosa的双二倍体与三个普通小麦栽培品种“德克”、“卡恩”和“坎撒其”回交而得的三个恢复系进行单体分析。“德克”的1A和7D染色体,“卡恩”的1A和6B染色体和“坎撒其”恢复系的6B与6D染色体均具有Rf。
The fertility restorer genes in six common wheat restorer lines were chromosomally located using a monomer set of male sterile “Kris” (Triticum aestivum) with Timofei cytoplasm. The six restorer lines were: R1-Lee (a derivative of Nebraska 542437 and the common wheat “Lee”), R2-Sonora 64 (Timofeev wheat and common wheat “Marquis” 3, a derivative of “Sonora 64”), R3 (a derivative of Timofeevi and ordinary wheat “Marquis” -3), R4 (Timofeev and Ae. Quarrosa Doubly diploid and derivatives of common wheat “Dirk” ~ 3), R5 (derivatives of Rokowski and common wheat “Justin” ~3), and common wheat “Puri”. The reciprocal ratio of male sterile and fertile plants of F 2 and F 3 derived from single F_1 showed that fertility restoration was found on chromosomes 1A and 7D of R1-Li, R2-Sonora 64, R3, R4 and R5 Gene (hereinafter referred to as Rf - Annotation). R2-Sonora 64 and R5 also each have a Rf, located on chromosomes 6B and 7B, respectively. “Primipi” has Rf on chromosomes 1B and 5D. Inheritance of the fertility recovery of the control and some non-discriminated derivatives shows that R1-Li, R2-Sonora 64 and R5 each have three Rf and R3 , R4, and “Puri” were each 2. The deletion of some chromosomes affected the penetrance and the expression of some of the derivatives, Rf.With several authors using monomer analysis to determine the Rf A chromosomal location. A restorer line (R3) obtained from the crossing of “Marquis” 3 with Timofevi wheat had an Rf on chromosome 1A and the second Rf was located on chromosome 7D of R3 Similarly, Talaat (1969) reported that R1-Li and R4 have Rf on 1A and 7D and a weak effect on 5A on 5A. The 1B chromosomes of R3 and R4 have a fertility-suppressing gene. However, There is a Rf on the 1B chromosome between “Duhamelianean” and the “Minnesota” wheat, which is dominated by the diploid with three diploids of the Timothy and Triticeps Ae, Squarrosa The three restorer lines of the common wheat cultivars “Dirk”, “Kahn” and “Kashiza” were backcrossed Analysis. “Dirk” the chromosome 1A and 7D, “Kahn” chromosome 6B and 1A, and the “Kansa its” chromosome 6B and 6D are restorer lines having Rf.