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以对羟基苯硼酸为前驱体,利用过氧化氢(H_2O_2)可以定量氧化对羟基苯硼酸产生对羟基苯酚的原理,以反应产物对羟基苯酚为电化学信号物质,结合金纳米粒子修饰玻碳电极(AuNPs/GCE),发展了一种间接检测H_2O_2的电化学方法.由于AuNPs/GCE具有有效电子传递性能和比表面积大等优点,对硼酸氧化产物具有较高的催化活性,因此在含1.0 mmol·L~(-1)对羟基苯硼酸的0.1 mol·L~(-1)pH 7.5 PBS中,AuNPs/GCE可以检测到1.0~1.0×10~3μmol·L~(-1)的H_2O_2,检测限为0.5μmol·L~(-1).同时,该方法具有良好的选择性和重现性,且操作简单、速度快、价格低廉,非常适用于实际样品中H_2O_2含量的测定.
Using p-hydroxybenzeneboronic acid as precursor, hydrogen peroxide (H_2O_2) can be used to quantitatively oxidize p-hydroxy-phenylboronic acid to p-hydroxybenzeneboronic acid. The reaction product of p-hydroxyphenol as electrochemical signal material, gold nanoparticles modified glassy carbon electrode (AuNPs / GCE), an indirect electrochemical method for the determination of H 2 O 2 has been developed. Since AuNPs / GCE has the advantages of effective electron transfer properties and large specific surface area, it has high catalytic activity for boric acid oxidation products. · AuNPs / GCE could detect 1.0 ~ 1.0 × 10 ~ 3μmol·L -1 H2O2 in 0.1 mol·L ~ (-1) pH 7.5 PBS with L ~ (-1) p-hydroxybenzeneboronic acid. The limit is 0.5μmol·L -1 .At the same time, the method has good selectivity and reproducibility, and is simple, fast and inexpensive, and is very suitable for the determination of H_2O_2 in real samples.