论文部分内容阅读
目的:研究和分离鉴定苦石莲的化学成分。方法:以苦石莲对牙周炎的关键因子白细胞介素-6(IL-6)和白细胞介素8(IL-8)的调控作用为依据,采取回流提取法和梯度萃取法进行提取分离,用反复硅胶柱色谱、薄层色谱、重结晶等方法分离纯化苦石莲的化学成分,通过质谱、核磁共振及理化常数对照等方法对化合物的结构进行鉴定。结果:分离鉴定了8个化合物,分别为豆甾醇(stigmasterol,1),caesalmin C(2),bonducellpin F(3),β-谷甾醇(β-sitsterol,4),β-香树脂醇(β-amyrin,5),正二十六烷(n-hexacosane,6),正三十烷(n-triacontane,7),西米杜鹃醇(simiarenol,8)。结论:化合物3,6,7,8为首次从苦石莲中分离得到,其中bonducellpin F为一个新的卡山烷型呋喃二萜类化合物,n-hexacosane和n-triacontane为脂肪烷烃类化合物,西米杜鹃醇为三萜类化合物。
Objective: To study and isolate and identify the chemical constituents of Kadsura. Methods: Based on the regulation of interleukin-6 (IL-6) and interleukin-8 (IL-8), the key factor of periodontitis in Kalanchoe-Palitiloneum L., reflux extraction and gradient extraction were used to extract and separate The chemical constituents were purified by repeated silica gel column chromatography, TLC and recrystallization. The structures of the compounds were identified by MS, NMR and physico-chemical constants. Results: Eight compounds were isolated and identified as stigmasterol (1), caesalmin C (2), bonducellpin F (3), β-sitsterol (4) -amyrin, 5), n-hexacosane (6), n-triacontane (7) and simiarenol (8). CONCLUSION: Compounds 3, 6, 7 and 8 were isolated from Kadsura lotus for the first time. Among them, bonducellpin F is a new kabane-type furan diterpenoid, n-hexacosane and n-triacontane are aliphatic alkanes, Symi Rhododendron alcohol is a triterpenoid.