Inhibition of Ampicillin-resistance in Bacteria by Modified DNAzymes

• 作 者: CHEN Fei LI Zhe YANG Shuo WANG
• 机 构: Key Lab for Molecular Enzymology and Engineering of the Ministry of Education,China-Japan Friendship
• 出 处: 高等学校化学研究（英文版）
• 发表日期: 2004年期
• 关键词: DNAzyme Drug-resistance β-Laetamase Ampicillin Antisense drug
• 基金项目: 国家自然科学基金

To overcome ampicillin-resistance of bacteria which is believed to attribute their endogenous β-lactamase,we designed three 10-23 DNAzymes(DZ1,Dz2,Dz3) targeting the coding region of β-lactamase mRNA and examined their inhibitory capabilities of the ampicillin-resistance of TEM-1 and TEM-3 bacteria,Dz1 was a traditional 10-23 DNAzyme,Dz2 was the mutant of Dz1 by addition of the protected nucleotide to each arm of the enzyme,and Dz3 was a mutant of DZ1 at antisense arms of which phosphorothioate modifications were made,Kinetic analysis,bacterial growth,and β-lactamase activity measurement showed that all the three DNAzymes worked efficiently in vitro and in vivo,A 9 hours bacterial growth inhibition test showed that the inhibition rates of TEM-1 bacteria by Dz1,Dz2,and Dz3 were 27%,50%,and 29%,respectively,In addition,the inhibition rates of TEM-3 bacteria by those three DNAzymes were found to be 49%,58%,and 45%,respectively,The current findings suggest that DNAzymes may become potential candidates of alternative inhibitors for bacteria drug-resistance.