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目的探讨转录因子T-bet/GATA3在肠道病毒71型(enterovirus 71,EV71)感染患儿外周血单个核细胞(peripheral blood mononuclear cell,PBMC)中的表达及其与相关细胞因子的关系。方法收集42例EV71感染患者外周血标本,其中急性期28例、恢复期14例,另外健康对照组21例。采用实时荧光定量PCR(QRT-PCR)检测PBMC中T-bet和GATA3 mRNA水平;酶联免疫吸附(ELISA)法检测血浆IFN-γ和IL-4的蛋白浓度。结果急性EV71感染患者PBMC中的T-bet mRNA表达和血浆IFN-γ水平明显低于健康对照组(P<0.05),急性EV71感染患者GATA3 mRNA的表达和IL-4水平则明显高于健康对照组(P<0.05)。结论急性EV71感染患者存在Th2优势分化,可能与转录因子T-bet表达下调、GATA3表达上调有关。监测EV71感染患者外周血Th1/Th2细胞特异性转录因子与相关细胞因子的水平,有助于对病情的判断。
Objective To investigate the expression of transcription factor T-bet / GATA3 in peripheral blood mononuclear cells (PBMC) infected with enterovirus 71 (EV71) and its relationship with related cytokines. Methods 42 peripheral blood samples from patients with EV71 infection were collected, of which 28 were acute and 14 were convalescent, while 21 were healthy controls. The levels of T-bet mRNA and GATA3 mRNA in PBMC were detected by real-time fluorescence quantitative PCR (QRT-PCR). The plasma concentrations of IFN-γ and IL-4 were detected by enzyme-linked immunosorbent assay (ELISA) Results The expression of T-bet mRNA and the level of IFN-γ in PBMC of patients with acute EV71 infection were significantly lower than those of healthy controls (P <0.05). The expression of GATA3 mRNA and the level of IL-4 in patients with acute EV71 infection were significantly higher than those in healthy controls Group (P <0.05). Conclusion Th2 predominant differentiation exists in patients with acute EV71 infection, which may be related to the down-regulation of transcription factor T-bet and upregulation of GATA3. Monitoring the level of Th1 / Th2 cell-specific transcription factors and related cytokines in the peripheral blood of patients with EV71 infection is helpful to judge the condition.